German cockroach extract induces activation of human eosinophils to release cytotoxic inflammatory mediators

Myung Hyun Sohn, Young Ah Lee, Kyeong Yong Jeong, Seobo Sim, Kyu Eun Kim, Taisoon Yong, Myeong Heon Shin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Eosinophils play an important role in the pathogenesis of allergic diseases. Sensitization and exposure to cockroach allergen have been demonstrated to be one of the major risk factors for the development of bronchial asthma. However, little is known regarding the functional capacity of cockroach extract antigen to activate human eosinophils. Objective: We investigated whether German cockroach extract can activate human eosinophils to release cytotoxic inflammatory mediators. Methods: Purified eosinophils from the peripheral blood were incubated with various concentrations (0-200 μg/ml) of German cockroach extract antigen. Effector functions of eosinophils were checked by degranulation and superoxide anion production. In addition, we examined surface expression of CD11b and CD69, and intracellular activation of p38 mitogen-activated protein kinase (p38 MAP kinase) in cockroach-stimulated eosinophils. Results: German cockroach extract induced degranulation and superoxide production from human eosinophils. In addition, incubation of eosinophils for 3 h with the cockroach extract resulted in an increased level of the surface expression of CD11b and CD69. Furthermore, cockroach-induced superoxide production from eosinophils was significantly inhibited by the pretreatment of cells with a p38 MAP kinase inhibitor SB202190. Indeed, a large amount of phosphorylated forms of p38 MAP kinase was detected in cockroach-stimulated eosinophils. Conclusions: Our results suggest that German cockroach extract induces activation of human eosinophils to release cytotoxic inflammatory mediators such as superoxide and granular proteins.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalInternational Archives of Allergy and Immunology
Volume134
Issue number2
DOIs
Publication statusPublished - 2004 Jun 14

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Blattellidae
Eosinophils
Cockroaches
Superoxides
p38 Mitogen-Activated Protein Kinases
Antigens
Protein Kinase Inhibitors
Allergens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Sohn, Myung Hyun ; Lee, Young Ah ; Jeong, Kyeong Yong ; Sim, Seobo ; Kim, Kyu Eun ; Yong, Taisoon ; Shin, Myeong Heon. / German cockroach extract induces activation of human eosinophils to release cytotoxic inflammatory mediators. In: International Archives of Allergy and Immunology. 2004 ; Vol. 134, No. 2. pp. 141-149.
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German cockroach extract induces activation of human eosinophils to release cytotoxic inflammatory mediators. / Sohn, Myung Hyun; Lee, Young Ah; Jeong, Kyeong Yong; Sim, Seobo; Kim, Kyu Eun; Yong, Taisoon; Shin, Myeong Heon.

In: International Archives of Allergy and Immunology, Vol. 134, No. 2, 14.06.2004, p. 141-149.

Research output: Contribution to journalArticle

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T1 - German cockroach extract induces activation of human eosinophils to release cytotoxic inflammatory mediators

AU - Sohn, Myung Hyun

AU - Lee, Young Ah

AU - Jeong, Kyeong Yong

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AU - Kim, Kyu Eun

AU - Yong, Taisoon

AU - Shin, Myeong Heon

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N2 - Background: Eosinophils play an important role in the pathogenesis of allergic diseases. Sensitization and exposure to cockroach allergen have been demonstrated to be one of the major risk factors for the development of bronchial asthma. However, little is known regarding the functional capacity of cockroach extract antigen to activate human eosinophils. Objective: We investigated whether German cockroach extract can activate human eosinophils to release cytotoxic inflammatory mediators. Methods: Purified eosinophils from the peripheral blood were incubated with various concentrations (0-200 μg/ml) of German cockroach extract antigen. Effector functions of eosinophils were checked by degranulation and superoxide anion production. In addition, we examined surface expression of CD11b and CD69, and intracellular activation of p38 mitogen-activated protein kinase (p38 MAP kinase) in cockroach-stimulated eosinophils. Results: German cockroach extract induced degranulation and superoxide production from human eosinophils. In addition, incubation of eosinophils for 3 h with the cockroach extract resulted in an increased level of the surface expression of CD11b and CD69. Furthermore, cockroach-induced superoxide production from eosinophils was significantly inhibited by the pretreatment of cells with a p38 MAP kinase inhibitor SB202190. Indeed, a large amount of phosphorylated forms of p38 MAP kinase was detected in cockroach-stimulated eosinophils. Conclusions: Our results suggest that German cockroach extract induces activation of human eosinophils to release cytotoxic inflammatory mediators such as superoxide and granular proteins.

AB - Background: Eosinophils play an important role in the pathogenesis of allergic diseases. Sensitization and exposure to cockroach allergen have been demonstrated to be one of the major risk factors for the development of bronchial asthma. However, little is known regarding the functional capacity of cockroach extract antigen to activate human eosinophils. Objective: We investigated whether German cockroach extract can activate human eosinophils to release cytotoxic inflammatory mediators. Methods: Purified eosinophils from the peripheral blood were incubated with various concentrations (0-200 μg/ml) of German cockroach extract antigen. Effector functions of eosinophils were checked by degranulation and superoxide anion production. In addition, we examined surface expression of CD11b and CD69, and intracellular activation of p38 mitogen-activated protein kinase (p38 MAP kinase) in cockroach-stimulated eosinophils. Results: German cockroach extract induced degranulation and superoxide production from human eosinophils. In addition, incubation of eosinophils for 3 h with the cockroach extract resulted in an increased level of the surface expression of CD11b and CD69. Furthermore, cockroach-induced superoxide production from eosinophils was significantly inhibited by the pretreatment of cells with a p38 MAP kinase inhibitor SB202190. Indeed, a large amount of phosphorylated forms of p38 MAP kinase was detected in cockroach-stimulated eosinophils. Conclusions: Our results suggest that German cockroach extract induces activation of human eosinophils to release cytotoxic inflammatory mediators such as superoxide and granular proteins.

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