TY - JOUR
T1 - GH-binding protein in obese men with varying glucose tolerance
T2 - Relationship to body fat distribution, insulin secretion and the GH-IGF-I axis
AU - Nam, Su Youn
AU - Kim, Kyung Rae
AU - Song, Young Duk
AU - Lim, Sung Kil
AU - Lee, Hyun Chul
AU - Huh, Kap Bum
PY - 1999/2
Y1 - 1999/2
N2 - Bioelectrical impedance for measurement of total body fat and computed tomography for visceral and subcutaneous fat at umbilicus levels were performed in 34 obese and 10 lean men. Insulin secretion in response to an oral glucose tolerance test (OGTT) and a GH stimulation test by L-dopa, growth hormone-binding protein (GHBP) and IGF-I were measured. Obese subjects were divided into three groups according to the OGTT. The obese type II diabetes mellitus group had the highest GHBP levels and the most visceral fat. GHBP levels were most strongly correlated with the ratio of visceral fat area to body weight (VWR) above any other parameters (r = 0.725, P < 0.001). The insulin and free fatty acid (FFA) areas under curves (AUC) during the OGTT, and the IGF-I level, were also positively correlated with GHBP levels (r=0.474, P<0.005; r=0.572, P<0.005; r=0.453, P<0.005). GH-AUC to the L-dopa stimulation test was negatively correlated with GHBP levels (r=-0.432, P < 0.005). Stepwise multiple linear regression analysis showed that VWR, FFA- AUC and insulin-AUC significantly contributed to the variability of GHBP (r2= 0.58). In conclusion, we demonstrated that: (i) visceral fat amount mainly determined GHBP levels in obese men with varying glucose tolerance; (ii) hyperglycemia per se did not influence the GHBP level, whereas insulin and FFA could play a role in regulation of GHBP; and (iii) although GH was not the main regulator of GHBP, the unchanged IGF-I level despite GH hyposecretion suggests that increased GHBP levels reflect GH hypersensitivity in order to compensate for decreased GH secretion in obesity.
AB - Bioelectrical impedance for measurement of total body fat and computed tomography for visceral and subcutaneous fat at umbilicus levels were performed in 34 obese and 10 lean men. Insulin secretion in response to an oral glucose tolerance test (OGTT) and a GH stimulation test by L-dopa, growth hormone-binding protein (GHBP) and IGF-I were measured. Obese subjects were divided into three groups according to the OGTT. The obese type II diabetes mellitus group had the highest GHBP levels and the most visceral fat. GHBP levels were most strongly correlated with the ratio of visceral fat area to body weight (VWR) above any other parameters (r = 0.725, P < 0.001). The insulin and free fatty acid (FFA) areas under curves (AUC) during the OGTT, and the IGF-I level, were also positively correlated with GHBP levels (r=0.474, P<0.005; r=0.572, P<0.005; r=0.453, P<0.005). GH-AUC to the L-dopa stimulation test was negatively correlated with GHBP levels (r=-0.432, P < 0.005). Stepwise multiple linear regression analysis showed that VWR, FFA- AUC and insulin-AUC significantly contributed to the variability of GHBP (r2= 0.58). In conclusion, we demonstrated that: (i) visceral fat amount mainly determined GHBP levels in obese men with varying glucose tolerance; (ii) hyperglycemia per se did not influence the GHBP level, whereas insulin and FFA could play a role in regulation of GHBP; and (iii) although GH was not the main regulator of GHBP, the unchanged IGF-I level despite GH hyposecretion suggests that increased GHBP levels reflect GH hypersensitivity in order to compensate for decreased GH secretion in obesity.
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U2 - 10.1530/eje.0.1400159
DO - 10.1530/eje.0.1400159
M3 - Article
C2 - 10069661
AN - SCOPUS:0345040221
VL - 140
SP - 159
EP - 163
JO - European Journal of Endocrinology
JF - European Journal of Endocrinology
SN - 0804-4643
IS - 2
ER -