Abstract
Giardia lamblia, a protozoan causing diarrheal outbreaks, is one of the main pathogens monitored in developed countries. Immunoscreening of G. lamblia expression library using the monoclonal antibodies (mAb) against G. lamblia, identified a subset of antigenic proteins in this protozoan, which are proteins belonging to GHSP (Giardia head-stalk protein), GHSP115, GHSP138, and GHSP180. In order to map the epitope region of GHSP115, the corresponding open reading frame was dissected into three parts and expressed as recombinant proteins with histidine tags. Western blot analysis of these recombinant proteins with mAbs reacting with GHSP115 indicated that one-third of the C-terminus of GHSP115 showed immunoreactivity with the mAb. Intracellular location of GHSP115 was examined both in trophozoites and encysting cells of G. lamblia by an immunofluorescence assay, indicating that location of GHSP115 varies during encystation. These results suggest that GHSP115 is an abundant and antigenic protein, which is differentially localized during life cycle of G. lamblia.
| Original language | English |
|---|---|
| Pages (from-to) | 11-16 |
| Number of pages | 6 |
| Journal | Experimental Parasitology |
| Volume | 122 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 May 1 |
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All Science Journal Classification (ASJC) codes
- Parasitology
- Immunology
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Giardia lamblia : Immunogenicity and intracellular distribution of GHSP-115, a member of the Giardia head-stalk family of proteins. / Bae, Sung Su; Kim, Juri; Kim, Tong Soo; Yong, Tai Soon; Park, Soon Jung.
In: Experimental Parasitology, Vol. 122, No. 1, 01.05.2009, p. 11-16.Research output: Contribution to journal › Article
TY - JOUR
T1 - Giardia lamblia
T2 - Immunogenicity and intracellular distribution of GHSP-115, a member of the Giardia head-stalk family of proteins
AU - Bae, Sung Su
AU - Kim, Juri
AU - Kim, Tong Soo
AU - Yong, Tai Soon
AU - Park, Soon Jung
PY - 2009/5/1
Y1 - 2009/5/1
N2 - Giardia lamblia, a protozoan causing diarrheal outbreaks, is one of the main pathogens monitored in developed countries. Immunoscreening of G. lamblia expression library using the monoclonal antibodies (mAb) against G. lamblia, identified a subset of antigenic proteins in this protozoan, which are proteins belonging to GHSP (Giardia head-stalk protein), GHSP115, GHSP138, and GHSP180. In order to map the epitope region of GHSP115, the corresponding open reading frame was dissected into three parts and expressed as recombinant proteins with histidine tags. Western blot analysis of these recombinant proteins with mAbs reacting with GHSP115 indicated that one-third of the C-terminus of GHSP115 showed immunoreactivity with the mAb. Intracellular location of GHSP115 was examined both in trophozoites and encysting cells of G. lamblia by an immunofluorescence assay, indicating that location of GHSP115 varies during encystation. These results suggest that GHSP115 is an abundant and antigenic protein, which is differentially localized during life cycle of G. lamblia.
AB - Giardia lamblia, a protozoan causing diarrheal outbreaks, is one of the main pathogens monitored in developed countries. Immunoscreening of G. lamblia expression library using the monoclonal antibodies (mAb) against G. lamblia, identified a subset of antigenic proteins in this protozoan, which are proteins belonging to GHSP (Giardia head-stalk protein), GHSP115, GHSP138, and GHSP180. In order to map the epitope region of GHSP115, the corresponding open reading frame was dissected into three parts and expressed as recombinant proteins with histidine tags. Western blot analysis of these recombinant proteins with mAbs reacting with GHSP115 indicated that one-third of the C-terminus of GHSP115 showed immunoreactivity with the mAb. Intracellular location of GHSP115 was examined both in trophozoites and encysting cells of G. lamblia by an immunofluorescence assay, indicating that location of GHSP115 varies during encystation. These results suggest that GHSP115 is an abundant and antigenic protein, which is differentially localized during life cycle of G. lamblia.
UR - http://www.scopus.com/inward/record.url?scp=64049099632&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64049099632&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2009.01.005
DO - 10.1016/j.exppara.2009.01.005
M3 - Article
C2 - 19545528
AN - SCOPUS:64049099632
VL - 122
SP - 11
EP - 16
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 1
ER -