Giardia lamblia: Immunogenicity and intracellular distribution of GHSP-115, a member of the Giardia head-stalk family of proteins

Sung Su Bae, Juri Kim, Tong Soo Kim, Tai Soon Yong, Soon Jung Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Giardia lamblia, a protozoan causing diarrheal outbreaks, is one of the main pathogens monitored in developed countries. Immunoscreening of G. lamblia expression library using the monoclonal antibodies (mAb) against G. lamblia, identified a subset of antigenic proteins in this protozoan, which are proteins belonging to GHSP (Giardia head-stalk protein), GHSP115, GHSP138, and GHSP180. In order to map the epitope region of GHSP115, the corresponding open reading frame was dissected into three parts and expressed as recombinant proteins with histidine tags. Western blot analysis of these recombinant proteins with mAbs reacting with GHSP115 indicated that one-third of the C-terminus of GHSP115 showed immunoreactivity with the mAb. Intracellular location of GHSP115 was examined both in trophozoites and encysting cells of G. lamblia by an immunofluorescence assay, indicating that location of GHSP115 varies during encystation. These results suggest that GHSP115 is an abundant and antigenic protein, which is differentially localized during life cycle of G. lamblia.

Original languageEnglish
Pages (from-to)11-16
Number of pages6
JournalExperimental Parasitology
Volume122
Issue number1
DOIs
Publication statusPublished - 2009 May 1

Fingerprint

Giardia
Giardia lamblia
Head
Proteins
Recombinant Proteins
Protozoan Proteins
Monoclonal Antibodies
Trophozoites
Life Cycle Stages
Histidine
Developed Countries
Open Reading Frames
Fluorescent Antibody Technique
Disease Outbreaks
Epitopes
Western Blotting

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Immunology

Cite this

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abstract = "Giardia lamblia, a protozoan causing diarrheal outbreaks, is one of the main pathogens monitored in developed countries. Immunoscreening of G. lamblia expression library using the monoclonal antibodies (mAb) against G. lamblia, identified a subset of antigenic proteins in this protozoan, which are proteins belonging to GHSP (Giardia head-stalk protein), GHSP115, GHSP138, and GHSP180. In order to map the epitope region of GHSP115, the corresponding open reading frame was dissected into three parts and expressed as recombinant proteins with histidine tags. Western blot analysis of these recombinant proteins with mAbs reacting with GHSP115 indicated that one-third of the C-terminus of GHSP115 showed immunoreactivity with the mAb. Intracellular location of GHSP115 was examined both in trophozoites and encysting cells of G. lamblia by an immunofluorescence assay, indicating that location of GHSP115 varies during encystation. These results suggest that GHSP115 is an abundant and antigenic protein, which is differentially localized during life cycle of G. lamblia.",
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Giardia lamblia : Immunogenicity and intracellular distribution of GHSP-115, a member of the Giardia head-stalk family of proteins. / Bae, Sung Su; Kim, Juri; Kim, Tong Soo; Yong, Tai Soon; Park, Soon Jung.

In: Experimental Parasitology, Vol. 122, No. 1, 01.05.2009, p. 11-16.

Research output: Contribution to journalArticle

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