Ginsenoside 20(S)-protopanaxatriol (PPT) activates peroxisome proliferator-activated receptor γ (PPARγ) in 3T3-L1 adipocytes

Kyu Lee Han, Myeong Ho Jung, Jong Hee Sohn, Jae-Kwan Hwang

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Peroxisome proliferator-activated receptor γ (PPARγ), a member of the nuclear receptor of ligand-activated transcription factors, regulates the expression of key genes involved in lipid and glucose metabolism or adipocyte differentiation. Ligands for this receptor have emerged as potent insulin sensitizers used in the treatment of Type2 diabetes. Ginseng saponins or ginsenosides are reported to provide anti-diabetic activity as well as to modulate glucose metabolism, although the mechanism remains unclear. In this study, we examined the effect of ginsenosides on activation of PPARγ and adipogenes in 3T3-L1. Using a GAL-4/PPARγ transactivation assay, 20(S)-protopanaxatriol (PPT), one of the ginsenoside metabolites, was found to increase PPARγ-transactivation activity dose-dependently with similar activity as troglitazone, a well-known PPARγ agonist. PPT enhanced adipogenesis by increasing the expression of PPARγ target genes such as aP2, LPL and PEPCK. Furthermore, PPT significantly increased expression of glucose transporter 4 (GLUT4). These results indicate that PPT can be developed as a PPARγ agonist for the improvement of insulin resistance associated with diabetes.

Original languageEnglish
Pages (from-to)110-113
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

Fingerprint

Ginsenosides
Peroxisome Proliferator-Activated Receptors
Adipocytes
troglitazone
Transcriptional Activation
Ligands
Glucose
Adipogenesis
Panax
Facilitative Glucose Transport Proteins
Saponins
Cytoplasmic and Nuclear Receptors
protopanaxatriol
Lipid Metabolism
Insulin Resistance
Transcription Factors
Insulin
Gene Expression

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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title = "Ginsenoside 20(S)-protopanaxatriol (PPT) activates peroxisome proliferator-activated receptor γ (PPARγ) in 3T3-L1 adipocytes",
abstract = "Peroxisome proliferator-activated receptor γ (PPARγ), a member of the nuclear receptor of ligand-activated transcription factors, regulates the expression of key genes involved in lipid and glucose metabolism or adipocyte differentiation. Ligands for this receptor have emerged as potent insulin sensitizers used in the treatment of Type2 diabetes. Ginseng saponins or ginsenosides are reported to provide anti-diabetic activity as well as to modulate glucose metabolism, although the mechanism remains unclear. In this study, we examined the effect of ginsenosides on activation of PPARγ and adipogenes in 3T3-L1. Using a GAL-4/PPARγ transactivation assay, 20(S)-protopanaxatriol (PPT), one of the ginsenoside metabolites, was found to increase PPARγ-transactivation activity dose-dependently with similar activity as troglitazone, a well-known PPARγ agonist. PPT enhanced adipogenesis by increasing the expression of PPARγ target genes such as aP2, LPL and PEPCK. Furthermore, PPT significantly increased expression of glucose transporter 4 (GLUT4). These results indicate that PPT can be developed as a PPARγ agonist for the improvement of insulin resistance associated with diabetes.",
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Ginsenoside 20(S)-protopanaxatriol (PPT) activates peroxisome proliferator-activated receptor γ (PPARγ) in 3T3-L1 adipocytes. / Han, Kyu Lee; Jung, Myeong Ho; Sohn, Jong Hee; Hwang, Jae-Kwan.

In: Biological and Pharmaceutical Bulletin, Vol. 29, No. 1, 01.01.2006, p. 110-113.

Research output: Contribution to journalArticle

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