Global Changes in Lipid Profiles of Mouse Cortex, Hippocampus, and Hypothalamus Upon p53 Knockout

Sang Tak Lee, Jong Cheol Lee, Jong Whi Kim, Soo Young Cho, Je Kyung Seong, Myeong Hee Moon

Research output: Contribution to journalArticle

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Abstract

Comprehensive lipidomic profiling in three different brain tissues (cortex, hippocampus, and hypothalamus) of mouse with p53 deficiency was performed by nanoflow liquid chromatography-tandem mass spectrometry (nLC-MS/MS) and the profile was compared with that of the wild type. p53 gene is a well-known tumour suppressor that prevents genome mutations that can cause cancers. More than 300 lipids (among 455 identified species), including phospholipids (PLs), sphingolipids, ceramides (Cers), and triacylglycerols (TAGs) were quantitatively analysed by selective reaction monitoring (SRM) of nanoflow ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (nUPLC-ESI-MS/MS). Among the three different neural tissues, hypothalamus demonstrated the most evident lipid profile changes upon p53 knockout. Alterations of PLs containing acyl chains of docosahexaenoic acid and arachidonic acid (highly enriched polyunsaturated fatty acids in the nervous system) were examined in relation to cell apoptosis upon p53 knockout. Comparison between sphingomyelins (SMs) and Cers showed that the conversion of SM to Cer did not effectively progress in the hypothalamus, resulting in the accumulation of SMs, possibly due to the inhibition of apoptosis caused by the lack of p53. Furthermore, TAGs were considerably decreased only in the hypothalamus, indicative of lipolysis that led to substantial weight loss of adipose tissue and muscles.

Original languageEnglish
Article number36510
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Nov 7

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Hypothalamus
Sphingomyelins
Hippocampus
Lipids
Ceramides
Tandem Mass Spectrometry
Liquid Chromatography
Phospholipids
Triglycerides
Apoptosis
Sphingolipids
Electrospray Ionization Mass Spectrometry
Docosahexaenoic Acids
Lipolysis
p53 Genes
Unsaturated Fatty Acids
Arachidonic Acid
Nervous System
Adipose Tissue
Weight Loss

All Science Journal Classification (ASJC) codes

  • General

Cite this

Lee, Sang Tak ; Lee, Jong Cheol ; Kim, Jong Whi ; Cho, Soo Young ; Seong, Je Kyung ; Moon, Myeong Hee. / Global Changes in Lipid Profiles of Mouse Cortex, Hippocampus, and Hypothalamus Upon p53 Knockout. In: Scientific reports. 2016 ; Vol. 6.
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Global Changes in Lipid Profiles of Mouse Cortex, Hippocampus, and Hypothalamus Upon p53 Knockout. / Lee, Sang Tak; Lee, Jong Cheol; Kim, Jong Whi; Cho, Soo Young; Seong, Je Kyung; Moon, Myeong Hee.

In: Scientific reports, Vol. 6, 36510, 07.11.2016.

Research output: Contribution to journalArticle

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