Global transcriptome analysis of eukaryotic genes affected by gromwell extract

Soohyun Bang, Dohyun Lee, Hanhe Kim, Jiyong Park, Yong-Sun Bahn

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

BACKGROUND: Gromwell is known to have diverse pharmacological, cosmetic and nutritional benefits for humans. Nevertheless, the biological influence of gromwell extract (GE) on the general physiology of eukaryotic cells remains unknown. In this study a global transcriptome analysis was performed to identify genes affected by the addition of GE with Cryptococcus neoformans as the model system. RESULTS: In response to GE treatment, genes involved in signal transduction were immediately regulated, and the evolutionarily conserved sets of genes involved in the core cellular functions, including DNA replication, RNA transcription/processing and protein translation/processing, were generally up-regulated. In contrast, a number of genes involved in carbohydrate metabolism and transport, inorganic ion transport and metabolism, post-translational modification/protein turnover/chaperone functions and signal transduction were down-regulated. Among the GE-responsive genes that are also evolutionarily conserved in the human genome, the expression patterns of YSA1, TPO2, CFO1 and PZF1 were confirmed by northern blot analysis. Based on the functional characterization of some GE-responsive genes, it was found that GE treatment may promote cellular tolerance against a variety of environmental stresses in eukaryotes. CONCLUSIONS: GE treatment affects the expression levels of a significant portion of the Cryptococcus genome, implying that GE significantly affects the general physiology of eukaryotic cells.

Original languageEnglish
Pages (from-to)445-452
Number of pages8
JournalJournal of the Science of Food and Agriculture
Volume94
Issue number3
DOIs
Publication statusPublished - 2014 Feb 1

Fingerprint

Lithospermum
Gene Expression Profiling
transcriptomics
extracts
Genes
genes
eukaryotic cells
Eukaryotic Cells
signal transduction
physiology
Signal Transduction
Cryptococcus (Tremellomycetes)
inorganic ions
genome
Cryptococcus neoformans
Cryptococcus
post-translational modification
ion transport
DNA replication
carbohydrate metabolism

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics

Cite this

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abstract = "BACKGROUND: Gromwell is known to have diverse pharmacological, cosmetic and nutritional benefits for humans. Nevertheless, the biological influence of gromwell extract (GE) on the general physiology of eukaryotic cells remains unknown. In this study a global transcriptome analysis was performed to identify genes affected by the addition of GE with Cryptococcus neoformans as the model system. RESULTS: In response to GE treatment, genes involved in signal transduction were immediately regulated, and the evolutionarily conserved sets of genes involved in the core cellular functions, including DNA replication, RNA transcription/processing and protein translation/processing, were generally up-regulated. In contrast, a number of genes involved in carbohydrate metabolism and transport, inorganic ion transport and metabolism, post-translational modification/protein turnover/chaperone functions and signal transduction were down-regulated. Among the GE-responsive genes that are also evolutionarily conserved in the human genome, the expression patterns of YSA1, TPO2, CFO1 and PZF1 were confirmed by northern blot analysis. Based on the functional characterization of some GE-responsive genes, it was found that GE treatment may promote cellular tolerance against a variety of environmental stresses in eukaryotes. CONCLUSIONS: GE treatment affects the expression levels of a significant portion of the Cryptococcus genome, implying that GE significantly affects the general physiology of eukaryotic cells.",
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Global transcriptome analysis of eukaryotic genes affected by gromwell extract. / Bang, Soohyun; Lee, Dohyun; Kim, Hanhe; Park, Jiyong; Bahn, Yong-Sun.

In: Journal of the Science of Food and Agriculture, Vol. 94, No. 3, 01.02.2014, p. 445-452.

Research output: Contribution to journalArticle

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