Glomerular mRNAS in human type 1 diabetes: Biochemical evidence for microalbuminuria as a manifestation of diabetic nephropathy

S. G. Adler, Shin-Wook Kang, S. Feld, Ryong Cha Dae Ryong Cha, L. Barba, L. Striker, G. Striker, B. L. Riser, J. LaPage, C. C. Nast

Research output: Contribution to journalArticle

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Abstract

Background. In patients with type 1 diabetes, some consider microalbuminuria to be a predictor of diabetic nephropathy while others believe it is an early feature of diabetic nephropathy. Methods. Levels of mRNAs that are of pathogenetic relevance in diabetic nephropathy were compared in glomeruli isolated from microalbuminuric and overtly proteinuric subjects and in control normoalbuminuric diabetic subjects and living renal transplant donors. Results. In subjects with microalbuminuria and overt proteinuria, glomerular mRNAs were virtually identical and approximately twofold higher for connective tissue growth factor (CTGF; P < 0.01) and collagen α2(IV) (P < 0.03) compared to living renal donors and normoalbuminuric patients. Glomerular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels were not significantly different among the groups (P = 0.4). Weak but statistically significant correlations were noted between CTGF mRNA and albuminuria (assessed by rank), fractional mesangial surface area, and a composite renal biopsy index. Glomerular CTGF mRNA correlated inversely with creatinine clearance. Glomerular collagen α2(IV) mRNA levels correlated with albuminuria (by rank) and less strongly with fractional mesangial area. Conclusion. To our knowledge, these data provide the first biochemical evidence demonstrating that the glomeruli of microalbuminuric patients and those with overt proteinuria do not differ significantly. The data support the concept that microalbuminuria is not "predictive" of diabetic nephropathy, but rather is an earlier point in the spectrum of diabetic nephropathy.

Original languageEnglish
Pages (from-to)2330-2336
Number of pages7
JournalKidney International
Volume60
Issue number6
DOIs
Publication statusPublished - 2001 Jan 1

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Diabetic Nephropathies
Type 1 Diabetes Mellitus
Messenger RNA
Albuminuria
Kidney
Proteinuria
Collagen
Connective Tissue Growth Factor
Glyceraldehyde-3-Phosphate Dehydrogenases
Living Donors
Creatinine
Tissue Donors
Biopsy

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Adler, S. G. ; Kang, Shin-Wook ; Feld, S. ; Dae Ryong Cha, Ryong Cha ; Barba, L. ; Striker, L. ; Striker, G. ; Riser, B. L. ; LaPage, J. ; Nast, C. C. / Glomerular mRNAS in human type 1 diabetes : Biochemical evidence for microalbuminuria as a manifestation of diabetic nephropathy. In: Kidney International. 2001 ; Vol. 60, No. 6. pp. 2330-2336.
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abstract = "Background. In patients with type 1 diabetes, some consider microalbuminuria to be a predictor of diabetic nephropathy while others believe it is an early feature of diabetic nephropathy. Methods. Levels of mRNAs that are of pathogenetic relevance in diabetic nephropathy were compared in glomeruli isolated from microalbuminuric and overtly proteinuric subjects and in control normoalbuminuric diabetic subjects and living renal transplant donors. Results. In subjects with microalbuminuria and overt proteinuria, glomerular mRNAs were virtually identical and approximately twofold higher for connective tissue growth factor (CTGF; P < 0.01) and collagen α2(IV) (P < 0.03) compared to living renal donors and normoalbuminuric patients. Glomerular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels were not significantly different among the groups (P = 0.4). Weak but statistically significant correlations were noted between CTGF mRNA and albuminuria (assessed by rank), fractional mesangial surface area, and a composite renal biopsy index. Glomerular CTGF mRNA correlated inversely with creatinine clearance. Glomerular collagen α2(IV) mRNA levels correlated with albuminuria (by rank) and less strongly with fractional mesangial area. Conclusion. To our knowledge, these data provide the first biochemical evidence demonstrating that the glomeruli of microalbuminuric patients and those with overt proteinuria do not differ significantly. The data support the concept that microalbuminuria is not {"}predictive{"} of diabetic nephropathy, but rather is an earlier point in the spectrum of diabetic nephropathy.",
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Adler, SG, Kang, S-W, Feld, S, Dae Ryong Cha, RC, Barba, L, Striker, L, Striker, G, Riser, BL, LaPage, J & Nast, CC 2001, 'Glomerular mRNAS in human type 1 diabetes: Biochemical evidence for microalbuminuria as a manifestation of diabetic nephropathy', Kidney International, vol. 60, no. 6, pp. 2330-2336. https://doi.org/10.1046/j.1523-1755.2001.00073.x

Glomerular mRNAS in human type 1 diabetes : Biochemical evidence for microalbuminuria as a manifestation of diabetic nephropathy. / Adler, S. G.; Kang, Shin-Wook; Feld, S.; Dae Ryong Cha, Ryong Cha; Barba, L.; Striker, L.; Striker, G.; Riser, B. L.; LaPage, J.; Nast, C. C.

In: Kidney International, Vol. 60, No. 6, 01.01.2001, p. 2330-2336.

Research output: Contribution to journalArticle

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T1 - Glomerular mRNAS in human type 1 diabetes

T2 - Biochemical evidence for microalbuminuria as a manifestation of diabetic nephropathy

AU - Adler, S. G.

AU - Kang, Shin-Wook

AU - Feld, S.

AU - Dae Ryong Cha, Ryong Cha

AU - Barba, L.

AU - Striker, L.

AU - Striker, G.

AU - Riser, B. L.

AU - LaPage, J.

AU - Nast, C. C.

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N2 - Background. In patients with type 1 diabetes, some consider microalbuminuria to be a predictor of diabetic nephropathy while others believe it is an early feature of diabetic nephropathy. Methods. Levels of mRNAs that are of pathogenetic relevance in diabetic nephropathy were compared in glomeruli isolated from microalbuminuric and overtly proteinuric subjects and in control normoalbuminuric diabetic subjects and living renal transplant donors. Results. In subjects with microalbuminuria and overt proteinuria, glomerular mRNAs were virtually identical and approximately twofold higher for connective tissue growth factor (CTGF; P < 0.01) and collagen α2(IV) (P < 0.03) compared to living renal donors and normoalbuminuric patients. Glomerular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels were not significantly different among the groups (P = 0.4). Weak but statistically significant correlations were noted between CTGF mRNA and albuminuria (assessed by rank), fractional mesangial surface area, and a composite renal biopsy index. Glomerular CTGF mRNA correlated inversely with creatinine clearance. Glomerular collagen α2(IV) mRNA levels correlated with albuminuria (by rank) and less strongly with fractional mesangial area. Conclusion. To our knowledge, these data provide the first biochemical evidence demonstrating that the glomeruli of microalbuminuric patients and those with overt proteinuria do not differ significantly. The data support the concept that microalbuminuria is not "predictive" of diabetic nephropathy, but rather is an earlier point in the spectrum of diabetic nephropathy.

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