Glycan microarrays from construction to applications

Yujun Kim, Ji Young Hyun, Injae Shin

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)


Through their specific interactions with proteins, cellular glycans play key roles in a wide range of physiological and pathological processes. One of the main goals of research in the areas of glycobiology and glycomedicine is to understand glycan-protein interactions at the molecular level. Over the past two decades, glycan microarrays have become powerful tools for the rapid evaluation of interactions between glycans and proteins. In this review, we briefly describe methods used for the preparation of glycan probes and the construction of glycan microarrays. Next, we highlight applications of glycan microarrays to rapid profiling of glycan-binding patterns of plant, animal and pathogenic lectins, as well as other proteins. Finally, we discuss other important uses of glycan microarrays, including the rapid analysis of substrate specificities of carbohydrate-active enzymes, the quantitative determination of glycan-protein interactions, discovering high-affinity or selective ligands for lectins, and identifying functional glycans within cells. We anticipate that this review will encourage researchers to employ glycan microarrays in diverse glycan-related studies.

Original languageEnglish
Pages (from-to)8276-8299
Number of pages24
JournalChemical Society reviews
Issue number19
Publication statusPublished - 2022 Sept 16

Bibliographical note

Funding Information:
This study was supported financially by the National Research Foundation of Korea (grant no. 2020R1A2C3003462 to I. S.) and the KRICT (grant no. SI-2231-30 and BSF22-914 to J. Y. H.).

Publisher Copyright:
© 2022 The Royal Society of Chemistry.

All Science Journal Classification (ASJC) codes

  • Chemistry(all)


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