Glycated albumin causes pancreatic β-cells dysfunction through autophagy dysfunction

Young Mi Song, Sun Ok Song, Young Hye You, Kun Ho Yoon, Eun Seok Kang, Bong Soo Cha, Hyun Chul Lee, Ji Won Kim, Byung Wan Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Growing evidence suggests that advanced glycation end-products (AGEs) are cytotoxic to pancreatic -cells. The aims of this study were to investigate whether glycated albumin (GA), an early precursor of AGEs, would induce dysfunction in pancreatic -cells and to determine which kinds of cellular mechanisms are activated in GA-induced-cell apoptosis. Decreased viability and increased apoptosis were induced in INS-1 cells treated with 2.5 mg/mL GA under 16.7mM high-glucose conditions. Insulin content and glucose-stimulated secretion from isolated rat islets were reduced in 2.5 mg/mL GA-treated cells. In response to 2.5 mg/mL GA in INS-1 cells, autophagy induction and flux decreased as assessed by green fluorescent protein-microtubule-associated protein 1 light chain 3 dots, microtubule-associated protein 1 light chain 3-II conversion, and SQSTM1/p62 in the presence and absence of bafilomycin A1. Accumulated SQSTM1/p62 through deficient autophagy activated the nuclear factor-B (p65)-inducible nitric oxide synthase-caspase-3 cascade, which was restored by treatment with small interfering RNA against p62. Small interfering RNA treatment against autophagy-related protein 5 significantly inhibited the autophagy machinery resulting in a significant increase in iNOS-cleaved caspase-3 expression. Treatment with 500-M 4-phenyl butyric acid significantly alleviated the expression of endoplasmic reticulum stress markers and iNOS in parallel with upregulated autophagy induction. However, in the presence of bafilomycin A1, the decreased viability of INS-1 cells was not recovered. Glycated albumin, an early precursor of AGE, caused pancreatic -cell death by inhibiting autophagy induction and flux, resulting in nuclear factor-B (p65)-iNOS-caspase-3 cascade activation as well as by increasing susceptibility to endoplasmic reticulum stress and oxidative stress.

Original languageEnglish
Pages (from-to)2626-2639
Number of pages14
JournalEndocrinology
Volume154
Issue number8
DOIs
Publication statusPublished - 2013 Aug 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Song, Y. M., Song, S. O., You, Y. H., Yoon, K. H., Kang, E. S., Cha, B. S., Lee, H. C., Kim, J. W., & Lee, B. W. (2013). Glycated albumin causes pancreatic β-cells dysfunction through autophagy dysfunction. Endocrinology, 154(8), 2626-2639. https://doi.org/10.1210/en.2013-1031