Gold-film array-electrode for electrochemical ELISA

S. D. Kim, J. W. Chung, J. T. Kim, H. Krause, J. C. Pyun

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18 Citations (Scopus)

Abstract

A gold-film array-electrode (8-channel) has been developed for application to an electrochemical ELISA system. The principle of the electrochemical ELISA is the amperometric quantification of oxidized 3,5,3′,5′- tetramethylbenzidine (TMB) which is widely used as a chromogenic substrate of horseradish peroxidase (HRP) in commercial ELISA kits. To make an 8-channel array-electrode, eight sets of three-electrode systems were integrated with the spacing of a 96-well microplate. The gold-film electrode for working- and auxiliary-electrode was made by sputtering a gold layer on a polyetherimide (PEI) strip, and the reference electrode was made by spin coating with Ag/AgCl paste. Using the gold-film electrode, the measurement parameters, such as analysis time, electrode area, measuring temperature and so on, were optimized. Using 50 array-electrodes, the electrode-to-electrode difference in sensitivity was estimated to be 8.2%. To demonstrate the practical applicability of the gold-film electrode, it was applied to commercial ELISA kits for the detection of HIV (antibody), HBV (antigen and antibody), and HCV (antibody). The life time of the gold-film electrode was estimated for use for over 500 repetitive measurements.

Original languageEnglish
Pages (from-to)463-469
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume111-112
Issue numberSUPPL.
DOIs
Publication statusPublished - 2005 Nov 11

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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