Abstract
Gold nanoparticles (d=10 nm) self-assembled with a specifically synthesized thiol-derivatized mannose were used to develop a novel colorimetric bioassay for glucose in human serum. The addition of Con A containing four binding sites induces the aggregation of mannose-stabilized gold nanoparticles via specific mannose-Con A interactions, which leads to a red shift of the surface plasmon absorption band centered at 520. nm. The presence of glucose in the solution hinders the Con A-induced aggregation of the mannose-stabilized gold nanoparticles because of the competitive binding of glucose to Con A. Thus, it results in a decrease of the surface plasmon absorption band shift. The simple surface plasmon absorption band change resulting from this method provides a selective means to detect and quantitate glucose in the linear concentration range of 500-4000μg/mL within 10. min with an experimental detection limit of 363μg/mL. The colorimetric assay was applied for the determination of glucose in human serum samples and the determined concentration was close to the value reported by the manufacturer of human serum sample used in the present experiment. The present bioassay can be successfully applied for the determination of human blood glucose levels for the diagnosis of diabetes without the use of enzymes.
| Original language | English |
|---|---|
| Pages (from-to) | 154-159 |
| Number of pages | 6 |
| Journal | Microchemical Journal |
| Volume | 106 |
| DOIs | |
| Publication status | Published - 2013 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Spectroscopy
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Gold glyconanoparticle-based colorimetric bioassay for the determination of glucose in human serum. / Lim, Kyung Rae; Park, Jong Myeon; Choi, Han Nim; Lee, Wonyong.
In: Microchemical Journal, Vol. 106, 01.01.2013, p. 154-159.Research output: Contribution to journal › Article
TY - JOUR
T1 - Gold glyconanoparticle-based colorimetric bioassay for the determination of glucose in human serum
AU - Lim, Kyung Rae
AU - Park, Jong Myeon
AU - Choi, Han Nim
AU - Lee, Wonyong
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Gold nanoparticles (d=10 nm) self-assembled with a specifically synthesized thiol-derivatized mannose were used to develop a novel colorimetric bioassay for glucose in human serum. The addition of Con A containing four binding sites induces the aggregation of mannose-stabilized gold nanoparticles via specific mannose-Con A interactions, which leads to a red shift of the surface plasmon absorption band centered at 520. nm. The presence of glucose in the solution hinders the Con A-induced aggregation of the mannose-stabilized gold nanoparticles because of the competitive binding of glucose to Con A. Thus, it results in a decrease of the surface plasmon absorption band shift. The simple surface plasmon absorption band change resulting from this method provides a selective means to detect and quantitate glucose in the linear concentration range of 500-4000μg/mL within 10. min with an experimental detection limit of 363μg/mL. The colorimetric assay was applied for the determination of glucose in human serum samples and the determined concentration was close to the value reported by the manufacturer of human serum sample used in the present experiment. The present bioassay can be successfully applied for the determination of human blood glucose levels for the diagnosis of diabetes without the use of enzymes.
AB - Gold nanoparticles (d=10 nm) self-assembled with a specifically synthesized thiol-derivatized mannose were used to develop a novel colorimetric bioassay for glucose in human serum. The addition of Con A containing four binding sites induces the aggregation of mannose-stabilized gold nanoparticles via specific mannose-Con A interactions, which leads to a red shift of the surface plasmon absorption band centered at 520. nm. The presence of glucose in the solution hinders the Con A-induced aggregation of the mannose-stabilized gold nanoparticles because of the competitive binding of glucose to Con A. Thus, it results in a decrease of the surface plasmon absorption band shift. The simple surface plasmon absorption band change resulting from this method provides a selective means to detect and quantitate glucose in the linear concentration range of 500-4000μg/mL within 10. min with an experimental detection limit of 363μg/mL. The colorimetric assay was applied for the determination of glucose in human serum samples and the determined concentration was close to the value reported by the manufacturer of human serum sample used in the present experiment. The present bioassay can be successfully applied for the determination of human blood glucose levels for the diagnosis of diabetes without the use of enzymes.
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UR - http://www.scopus.com/inward/citedby.url?scp=84868303465&partnerID=8YFLogxK
U2 - 10.1016/j.microc.2012.06.003
DO - 10.1016/j.microc.2012.06.003
M3 - Article
VL - 106
SP - 154
EP - 159
JO - Microchemical Journal
JF - Microchemical Journal
SN - 0026-265X
ER -