Gold-nanoparticle-coated magnetic beads for concentration and ionization of analytes for laser desorption/ionization mass spectrometry

Moon Ju Kim, Jong Min Park, Joo Yoon Noh, Tae Gyeong Yun, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Rationale: Magnetic particles coated with gold nanoparticles (Au NPs) (Au-MAGs) were developed and used (1) for sample concentration and (2) as a solid matrix for laser adsorption/desorption mass spectrometry (LDI-MS). Methods: The Au-MAGs were prepared by (1) coating polystyrene on iron oxide NPs (PS-MNPs), (2) coating poly-l-lysine on the PS-MNPs (PLL-coated PS-MNPs), and (3) coating negatively charged Au NPs on the PLL-coated PS-MNPs (Au-MAGs). Results: The Au-MAGs were used to concentrate the target analyte by means of electrostatic interactions between the positively charged GHP9 and the negatively charged Au-MAGs as well as selective interactions (such as gold–sulfur interactions) between glutathione (GSH) and Au-MAGs. Then, the concentrated analyte could be ionized for LDI-MS. Conclusions: The Au-MAGs were demonstrated (1) to concentrate the target analyte in a sample solution, tested by electrostatic interactions and selective interactions between gold and sulfur and (2) to ionize the concentrated analyte for LDI-MS.

Original languageEnglish
Pages (from-to)527-538
Number of pages12
JournalRapid Communications in Mass Spectrometry
Volume33
Issue number5
DOIs
Publication statusPublished - 2019 Mar 15

Bibliographical note

Funding Information:
This work was supported by the Nano‐Convergence Foundation (Grant No. R201602210) funded by the Ministry of Science, ICT and Future Planning (MSIP, Korea) and Ministry of Trade, Industry and Energy (MOTIE, Korea), Industry Technology Development Program [grant number: 10063335] funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea), and National Research Foundation of Korea (Grant Nos. NRF‐2017R1A2B4004077 and NRF‐2017R1A2B2004398).

Funding Information:
Industry Technology Development Program, Grant/Award Number: 10063335; National Research Foundation of Korea, Grant/Award Numbers: NRF‐2017R1A2B2004398 and NRF‐2017R1A2B4004077; Ministry of Trade, Industry and Energy (MOTIE, Korea); Ministry of Science, ICT and Future Planning (MSIP, Korea); Nano‐Convergence Foundation, Grant/Award Number: R201602210

Publisher Copyright:
© 2018 John Wiley & Sons, Ltd.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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