Gold nanoparticles enhance anti-tumor effect of radiotherapy to hypoxic tumor

Mi Sun Kim, Eun Jung Lee, Jae Won Kim, Ui Seok Chung, Won Gun Koh, Ki Chang Keum, Woong Sub Koom

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: Hypoxia can impair the therapeutic efficacy of radiotherapy (RT). Therefore, a new strategy is necessary for enhancing the response to RT. In this study, we investigated whether the combination of nanoparticles and RT is effective in eliminating the radioresistance of hypoxic tumors. Materials and Methods: Gold nanoparticles (GNPs) consisting of a silica core with a gold shell were used. CT26 colon cancer mouse model was developed to study whether the combination of RT and GNPs reduced hypoxia-induced radioresistance. Hypoxia inducible factor-1a (HIF-1a) was used as a hypoxia marker. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were conducted to evaluate cell death. Results: Hypoxic tumor cells had an impaired response to RT. GNPs combined with RT enhanced anti-tumor effect in hypoxic tumor compared with RT alone. The combination of GNPs and RT decreased tumor cell viability compare to RT alone in vitro. Under hypoxia, tumors treated with GNPs + RT showed a higher response than that shown by tumors treated with RT alone. When a reactive oxygen species (ROS) scavenger was added, the enhanced antitumor effect of GNPs + RT was diminished. Conclusion: In the present study, hypoxic tumors treated with GNPs + RT showed favorable responses, which might be attributable to the ROS production induced by GNPs + RT. Taken together, GNPs combined with RT seems to be potential modality for enhancing the response to RT in hypoxic tumors.

Original languageEnglish
Pages (from-to)230-238
Number of pages9
JournalRadiation Oncology Journal
Volume34
Issue number3
DOIs
Publication statusPublished - 2016 Sep

Fingerprint

Gold
Nanoparticles
Radiotherapy
Neoplasms
Reactive Oxygen Species
DNA Nucleotidylexotransferase
Silicon Dioxide
Colonic Neoplasms
Cell Survival
Cell Death

All Science Journal Classification (ASJC) codes

  • Oncology
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, M. S., Lee, E. J., Kim, J. W., Chung, U. S., Koh, W. G., Keum, K. C., & Koom, W. S. (2016). Gold nanoparticles enhance anti-tumor effect of radiotherapy to hypoxic tumor. Radiation Oncology Journal, 34(3), 230-238. https://doi.org/10.3857/roj.2016.01788
Kim, Mi Sun ; Lee, Eun Jung ; Kim, Jae Won ; Chung, Ui Seok ; Koh, Won Gun ; Keum, Ki Chang ; Koom, Woong Sub. / Gold nanoparticles enhance anti-tumor effect of radiotherapy to hypoxic tumor. In: Radiation Oncology Journal. 2016 ; Vol. 34, No. 3. pp. 230-238.
@article{efb707dc7a2541eb93308eea69db913f,
title = "Gold nanoparticles enhance anti-tumor effect of radiotherapy to hypoxic tumor",
abstract = "Purpose: Hypoxia can impair the therapeutic efficacy of radiotherapy (RT). Therefore, a new strategy is necessary for enhancing the response to RT. In this study, we investigated whether the combination of nanoparticles and RT is effective in eliminating the radioresistance of hypoxic tumors. Materials and Methods: Gold nanoparticles (GNPs) consisting of a silica core with a gold shell were used. CT26 colon cancer mouse model was developed to study whether the combination of RT and GNPs reduced hypoxia-induced radioresistance. Hypoxia inducible factor-1a (HIF-1a) was used as a hypoxia marker. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were conducted to evaluate cell death. Results: Hypoxic tumor cells had an impaired response to RT. GNPs combined with RT enhanced anti-tumor effect in hypoxic tumor compared with RT alone. The combination of GNPs and RT decreased tumor cell viability compare to RT alone in vitro. Under hypoxia, tumors treated with GNPs + RT showed a higher response than that shown by tumors treated with RT alone. When a reactive oxygen species (ROS) scavenger was added, the enhanced antitumor effect of GNPs + RT was diminished. Conclusion: In the present study, hypoxic tumors treated with GNPs + RT showed favorable responses, which might be attributable to the ROS production induced by GNPs + RT. Taken together, GNPs combined with RT seems to be potential modality for enhancing the response to RT in hypoxic tumors.",
author = "Kim, {Mi Sun} and Lee, {Eun Jung} and Kim, {Jae Won} and Chung, {Ui Seok} and Koh, {Won Gun} and Keum, {Ki Chang} and Koom, {Woong Sub}",
year = "2016",
month = "9",
doi = "10.3857/roj.2016.01788",
language = "English",
volume = "34",
pages = "230--238",
journal = "Radiation Oncology Journal",
issn = "2234-1900",
publisher = "Korean Society for Therapeutic Radiology and Oncology",
number = "3",

}

Gold nanoparticles enhance anti-tumor effect of radiotherapy to hypoxic tumor. / Kim, Mi Sun; Lee, Eun Jung; Kim, Jae Won; Chung, Ui Seok; Koh, Won Gun; Keum, Ki Chang; Koom, Woong Sub.

In: Radiation Oncology Journal, Vol. 34, No. 3, 09.2016, p. 230-238.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Gold nanoparticles enhance anti-tumor effect of radiotherapy to hypoxic tumor

AU - Kim, Mi Sun

AU - Lee, Eun Jung

AU - Kim, Jae Won

AU - Chung, Ui Seok

AU - Koh, Won Gun

AU - Keum, Ki Chang

AU - Koom, Woong Sub

PY - 2016/9

Y1 - 2016/9

N2 - Purpose: Hypoxia can impair the therapeutic efficacy of radiotherapy (RT). Therefore, a new strategy is necessary for enhancing the response to RT. In this study, we investigated whether the combination of nanoparticles and RT is effective in eliminating the radioresistance of hypoxic tumors. Materials and Methods: Gold nanoparticles (GNPs) consisting of a silica core with a gold shell were used. CT26 colon cancer mouse model was developed to study whether the combination of RT and GNPs reduced hypoxia-induced radioresistance. Hypoxia inducible factor-1a (HIF-1a) was used as a hypoxia marker. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were conducted to evaluate cell death. Results: Hypoxic tumor cells had an impaired response to RT. GNPs combined with RT enhanced anti-tumor effect in hypoxic tumor compared with RT alone. The combination of GNPs and RT decreased tumor cell viability compare to RT alone in vitro. Under hypoxia, tumors treated with GNPs + RT showed a higher response than that shown by tumors treated with RT alone. When a reactive oxygen species (ROS) scavenger was added, the enhanced antitumor effect of GNPs + RT was diminished. Conclusion: In the present study, hypoxic tumors treated with GNPs + RT showed favorable responses, which might be attributable to the ROS production induced by GNPs + RT. Taken together, GNPs combined with RT seems to be potential modality for enhancing the response to RT in hypoxic tumors.

AB - Purpose: Hypoxia can impair the therapeutic efficacy of radiotherapy (RT). Therefore, a new strategy is necessary for enhancing the response to RT. In this study, we investigated whether the combination of nanoparticles and RT is effective in eliminating the radioresistance of hypoxic tumors. Materials and Methods: Gold nanoparticles (GNPs) consisting of a silica core with a gold shell were used. CT26 colon cancer mouse model was developed to study whether the combination of RT and GNPs reduced hypoxia-induced radioresistance. Hypoxia inducible factor-1a (HIF-1a) was used as a hypoxia marker. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were conducted to evaluate cell death. Results: Hypoxic tumor cells had an impaired response to RT. GNPs combined with RT enhanced anti-tumor effect in hypoxic tumor compared with RT alone. The combination of GNPs and RT decreased tumor cell viability compare to RT alone in vitro. Under hypoxia, tumors treated with GNPs + RT showed a higher response than that shown by tumors treated with RT alone. When a reactive oxygen species (ROS) scavenger was added, the enhanced antitumor effect of GNPs + RT was diminished. Conclusion: In the present study, hypoxic tumors treated with GNPs + RT showed favorable responses, which might be attributable to the ROS production induced by GNPs + RT. Taken together, GNPs combined with RT seems to be potential modality for enhancing the response to RT in hypoxic tumors.

UR - http://www.scopus.com/inward/record.url?scp=84990996804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990996804&partnerID=8YFLogxK

U2 - 10.3857/roj.2016.01788

DO - 10.3857/roj.2016.01788

M3 - Article

AN - SCOPUS:84990996804

VL - 34

SP - 230

EP - 238

JO - Radiation Oncology Journal

JF - Radiation Oncology Journal

SN - 2234-1900

IS - 3

ER -