GPA-9 is a novel regulator of innate immunity against Escherichia coli foods in adult Caenorhabditis elegans

Jeong Hoon Hahm, Sunhee Kim, Young-Ki Paik

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Innate immune responses to pathogens are governed by the nervous system. Here, we investigated the molecular mechanism underlying innate immunity in Caenorhabditis elegans against Escherichia coli OP50, a standard laboratory C. elegans food. Longevity was compared in worms fed live or UV-killed OP50 at low or high density food condition (HDF). Expression of the antimicrobial gene lys-8 was approximately 5-fold higher in worms fed live OP50, suggesting activation of innate immunity upon recognition of OP50 metabolites. Lifespan was extended and SOD-3 mRNA levels were increased in gpa-9-overexpressing gpa-9XS worms under HDF in association with robust induction of insulin/IGF-1 signaling (IIS). Expression of ins-7 and daf-28 that control lys-8 expression was reduced in gpa-9XS, indicating that GPA-9-mediated immunity is due in part to ins-7 and daf-28 downregulation. Our results suggest that OP50 metabolites in amphid neurons elicit innate immunity through the IIS pathway, and identify GPA-9 as a novel regulator of both the immune system and aging in C. elegans.

Original languageEnglish
Pages (from-to)208-219
Number of pages12
JournalAging Cell
Volume10
Issue number2
DOIs
Publication statusPublished - 2011 Apr 1

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Caenorhabditis elegans
Innate Immunity
Escherichia coli
Food
Insulin-Like Growth Factor I
Insulin
Nervous System
Immune System
Immunity
Down-Regulation
Gene Expression
Neurons
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Ageing
  • Cell Biology

Cite this

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GPA-9 is a novel regulator of innate immunity against Escherichia coli foods in adult Caenorhabditis elegans. / Hahm, Jeong Hoon; Kim, Sunhee; Paik, Young-Ki.

In: Aging Cell, Vol. 10, No. 2, 01.04.2011, p. 208-219.

Research output: Contribution to journalArticle

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