GPA-9 is a novel regulator of innate immunity against Escherichia coli foods in adult Caenorhabditis elegans

Jeong Hoon Hahm, Sunhee Kim, Young Ki Paik

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13 Citations (Scopus)


Innate immune responses to pathogens are governed by the nervous system. Here, we investigated the molecular mechanism underlying innate immunity in Caenorhabditis elegans against Escherichia coli OP50, a standard laboratory C. elegans food. Longevity was compared in worms fed live or UV-killed OP50 at low or high density food condition (HDF). Expression of the antimicrobial gene lys-8 was approximately 5-fold higher in worms fed live OP50, suggesting activation of innate immunity upon recognition of OP50 metabolites. Lifespan was extended and SOD-3 mRNA levels were increased in gpa-9-overexpressing gpa-9XS worms under HDF in association with robust induction of insulin/IGF-1 signaling (IIS). Expression of ins-7 and daf-28 that control lys-8 expression was reduced in gpa-9XS, indicating that GPA-9-mediated immunity is due in part to ins-7 and daf-28 downregulation. Our results suggest that OP50 metabolites in amphid neurons elicit innate immunity through the IIS pathway, and identify GPA-9 as a novel regulator of both the immune system and aging in C. elegans.

Original languageEnglish
Pages (from-to)208-219
Number of pages12
JournalAging Cell
Issue number2
Publication statusPublished - 2011 Apr 1


All Science Journal Classification (ASJC) codes

  • Ageing
  • Cell Biology

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