Graded functionalization of biomaterial surfaces using mussel-inspired adhesive coating of polydopamine

Sajeesh Kumar Madhurakkat Perikamana, Young Min Shin, Jin Kyu Lee, Yu Bin Lee, Yunhoe Heo, Taufiq Ahmad, So Yeon Park, Jisoo Shin, Kyung Min Park, Hyun Suk Jung, Seung-Woo Cho, Heungsoo Shin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Biomaterials with graded functionality have various applications in cell and tissue engineering. In this study, by controlling oxidative polymerization of dopamine, we demonstrated universal techniques for generating chemical gradients on various materials with adaptability for secondary molecule immobilization. Diffusion-controlled oxygen supply was successfully exploited for coating of polydopamine (PD) in a gradient manner on different materials, regardless of their surface chemistry, which resulted in gradient in hydrophilicity and surface roughness. The PD gradient controlled graded adhesion and spreading of human mesenchymal stem cells (hMSCs) and endothelial cells. Furthermore, the PD gradient on these surfaces served as a template to allow for graded immobilization of different secondary biomolecules such as cell adhesive arginine-glycine-aspartate (RGD) peptides and siRNA lipidoid nanoparticles (sLNP) complex, for site-specific adhesion of human mesenchymal stem cells, and silencing of green fluorescent protein (GFP) expression on GFP-HeLa cells, respectively. In addition, the same approach was adapted for generation of nanofibers with surface in graded biomineralization under simulated body fluid (SBF). Collectively, oxygen-dependent generation of PD gradient on biomaterial substrates can serve as a simple and versatile platform that can be used for various applications realizing in vivo tissue regeneration and in vitro high-throughput screening of biomaterials.

Original languageEnglish
Pages (from-to)546-556
Number of pages11
JournalColloids and Surfaces B: Biointerfaces
Volume159
DOIs
Publication statusPublished - 2017 Nov 1

Fingerprint

Bivalvia
Biocompatible Materials
Biomaterials
Adhesives
adhesives
Stem cells
coatings
Coatings
gradients
Adhesion
Green Fluorescent Proteins
Cell engineering
Mesenchymal Stromal Cells
Immobilization
Proteins
Biomineralization
Oxygen supply
Tissue regeneration
Arginine
stem cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Perikamana, Sajeesh Kumar Madhurakkat ; Shin, Young Min ; Lee, Jin Kyu ; Lee, Yu Bin ; Heo, Yunhoe ; Ahmad, Taufiq ; Park, So Yeon ; Shin, Jisoo ; Park, Kyung Min ; Jung, Hyun Suk ; Cho, Seung-Woo ; Shin, Heungsoo. / Graded functionalization of biomaterial surfaces using mussel-inspired adhesive coating of polydopamine. In: Colloids and Surfaces B: Biointerfaces. 2017 ; Vol. 159. pp. 546-556.
@article{fd54b0bf80f94c98a16e93baeeac8641,
title = "Graded functionalization of biomaterial surfaces using mussel-inspired adhesive coating of polydopamine",
abstract = "Biomaterials with graded functionality have various applications in cell and tissue engineering. In this study, by controlling oxidative polymerization of dopamine, we demonstrated universal techniques for generating chemical gradients on various materials with adaptability for secondary molecule immobilization. Diffusion-controlled oxygen supply was successfully exploited for coating of polydopamine (PD) in a gradient manner on different materials, regardless of their surface chemistry, which resulted in gradient in hydrophilicity and surface roughness. The PD gradient controlled graded adhesion and spreading of human mesenchymal stem cells (hMSCs) and endothelial cells. Furthermore, the PD gradient on these surfaces served as a template to allow for graded immobilization of different secondary biomolecules such as cell adhesive arginine-glycine-aspartate (RGD) peptides and siRNA lipidoid nanoparticles (sLNP) complex, for site-specific adhesion of human mesenchymal stem cells, and silencing of green fluorescent protein (GFP) expression on GFP-HeLa cells, respectively. In addition, the same approach was adapted for generation of nanofibers with surface in graded biomineralization under simulated body fluid (SBF). Collectively, oxygen-dependent generation of PD gradient on biomaterial substrates can serve as a simple and versatile platform that can be used for various applications realizing in vivo tissue regeneration and in vitro high-throughput screening of biomaterials.",
author = "Perikamana, {Sajeesh Kumar Madhurakkat} and Shin, {Young Min} and Lee, {Jin Kyu} and Lee, {Yu Bin} and Yunhoe Heo and Taufiq Ahmad and Park, {So Yeon} and Jisoo Shin and Park, {Kyung Min} and Jung, {Hyun Suk} and Seung-Woo Cho and Heungsoo Shin",
year = "2017",
month = "11",
day = "1",
doi = "10.1016/j.colsurfb.2017.08.022",
language = "English",
volume = "159",
pages = "546--556",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

Perikamana, SKM, Shin, YM, Lee, JK, Lee, YB, Heo, Y, Ahmad, T, Park, SY, Shin, J, Park, KM, Jung, HS, Cho, S-W & Shin, H 2017, 'Graded functionalization of biomaterial surfaces using mussel-inspired adhesive coating of polydopamine', Colloids and Surfaces B: Biointerfaces, vol. 159, pp. 546-556. https://doi.org/10.1016/j.colsurfb.2017.08.022

Graded functionalization of biomaterial surfaces using mussel-inspired adhesive coating of polydopamine. / Perikamana, Sajeesh Kumar Madhurakkat; Shin, Young Min; Lee, Jin Kyu; Lee, Yu Bin; Heo, Yunhoe; Ahmad, Taufiq; Park, So Yeon; Shin, Jisoo; Park, Kyung Min; Jung, Hyun Suk; Cho, Seung-Woo; Shin, Heungsoo.

In: Colloids and Surfaces B: Biointerfaces, Vol. 159, 01.11.2017, p. 546-556.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Graded functionalization of biomaterial surfaces using mussel-inspired adhesive coating of polydopamine

AU - Perikamana, Sajeesh Kumar Madhurakkat

AU - Shin, Young Min

AU - Lee, Jin Kyu

AU - Lee, Yu Bin

AU - Heo, Yunhoe

AU - Ahmad, Taufiq

AU - Park, So Yeon

AU - Shin, Jisoo

AU - Park, Kyung Min

AU - Jung, Hyun Suk

AU - Cho, Seung-Woo

AU - Shin, Heungsoo

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Biomaterials with graded functionality have various applications in cell and tissue engineering. In this study, by controlling oxidative polymerization of dopamine, we demonstrated universal techniques for generating chemical gradients on various materials with adaptability for secondary molecule immobilization. Diffusion-controlled oxygen supply was successfully exploited for coating of polydopamine (PD) in a gradient manner on different materials, regardless of their surface chemistry, which resulted in gradient in hydrophilicity and surface roughness. The PD gradient controlled graded adhesion and spreading of human mesenchymal stem cells (hMSCs) and endothelial cells. Furthermore, the PD gradient on these surfaces served as a template to allow for graded immobilization of different secondary biomolecules such as cell adhesive arginine-glycine-aspartate (RGD) peptides and siRNA lipidoid nanoparticles (sLNP) complex, for site-specific adhesion of human mesenchymal stem cells, and silencing of green fluorescent protein (GFP) expression on GFP-HeLa cells, respectively. In addition, the same approach was adapted for generation of nanofibers with surface in graded biomineralization under simulated body fluid (SBF). Collectively, oxygen-dependent generation of PD gradient on biomaterial substrates can serve as a simple and versatile platform that can be used for various applications realizing in vivo tissue regeneration and in vitro high-throughput screening of biomaterials.

AB - Biomaterials with graded functionality have various applications in cell and tissue engineering. In this study, by controlling oxidative polymerization of dopamine, we demonstrated universal techniques for generating chemical gradients on various materials with adaptability for secondary molecule immobilization. Diffusion-controlled oxygen supply was successfully exploited for coating of polydopamine (PD) in a gradient manner on different materials, regardless of their surface chemistry, which resulted in gradient in hydrophilicity and surface roughness. The PD gradient controlled graded adhesion and spreading of human mesenchymal stem cells (hMSCs) and endothelial cells. Furthermore, the PD gradient on these surfaces served as a template to allow for graded immobilization of different secondary biomolecules such as cell adhesive arginine-glycine-aspartate (RGD) peptides and siRNA lipidoid nanoparticles (sLNP) complex, for site-specific adhesion of human mesenchymal stem cells, and silencing of green fluorescent protein (GFP) expression on GFP-HeLa cells, respectively. In addition, the same approach was adapted for generation of nanofibers with surface in graded biomineralization under simulated body fluid (SBF). Collectively, oxygen-dependent generation of PD gradient on biomaterial substrates can serve as a simple and versatile platform that can be used for various applications realizing in vivo tissue regeneration and in vitro high-throughput screening of biomaterials.

UR - http://www.scopus.com/inward/record.url?scp=85028051219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028051219&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2017.08.022

DO - 10.1016/j.colsurfb.2017.08.022

M3 - Article

C2 - 28850919

AN - SCOPUS:85028051219

VL - 159

SP - 546

EP - 556

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -