Graft copolymer-templated mesoporous TiO2 films micropatterned with poly(ethylene glycol) hydrogel: Novel platform for highly sensitive protein microarrays

Kyung Jin Son, Sung Hoon Ahn, Jong Hak Kim, Won Gun Koh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this study, we describe the use of organized mesoporous titaniumoxide (TiO2) films as three-dimensional templates for protein microarrays with enhanced protein loading capacity and detection sensitivity. Multilayered mesoporous TiO2 films with high porosity and good connectivity were synthesized using a graft copolymer consisting of a poly(vinyl chloride) (PVC) backbone and poly(oxyethylene methacrylate) (POEM) side chains as a structure-directing template. The average pore size and thickness of the TiO2 films were 50-70 nm and 1.5 μm, respectively. Proteins were covalently immobilized onto mesoporous TiO2 film via 3-aminopropyltriethoxysilane (APTES), and protein loading onto TiO2 films was about four times greater than on planar glass substrates, which consequently improved the protein activity. Micropatterned mesoporous TiO 2 substrates were prepared by fabricating poly(ethylene glycol) (PEG) hydrogel microstructures on TiO2 films using photolithography. Because of nonadhesiveness of PEG hydrogel towards proteins, proteins were selectively immobilized onto surface-modified mesoporous TiO2 region, creating protein microarray. Specific binding assay between streptavidin/biotin and between PSA/anti-PSA demonstrated that the mesoporous TiO2-based protein microarrays yielded higher fluorescence signals and were more sensitive with lower detection limits than microarrays based on planar glass slides.

Original languageEnglish
Pages (from-to)573-581
Number of pages9
JournalACS Applied Materials and Interfaces
Volume3
Issue number2
DOIs
Publication statusPublished - 2011 Feb 23

Fingerprint

Hydrogel
Graft copolymers
Microarrays
Hydrogels
Polyethylene glycols
Proteins
Vinyl Chloride
Glass
Streptavidin
Methacrylates
Photolithography
Substrates
Biotin
Polyvinyl Chloride
Polyvinyl chlorides
Pore size
Assays
Porosity
Fluorescence
Microstructure

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

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abstract = "In this study, we describe the use of organized mesoporous titaniumoxide (TiO2) films as three-dimensional templates for protein microarrays with enhanced protein loading capacity and detection sensitivity. Multilayered mesoporous TiO2 films with high porosity and good connectivity were synthesized using a graft copolymer consisting of a poly(vinyl chloride) (PVC) backbone and poly(oxyethylene methacrylate) (POEM) side chains as a structure-directing template. The average pore size and thickness of the TiO2 films were 50-70 nm and 1.5 μm, respectively. Proteins were covalently immobilized onto mesoporous TiO2 film via 3-aminopropyltriethoxysilane (APTES), and protein loading onto TiO2 films was about four times greater than on planar glass substrates, which consequently improved the protein activity. Micropatterned mesoporous TiO 2 substrates were prepared by fabricating poly(ethylene glycol) (PEG) hydrogel microstructures on TiO2 films using photolithography. Because of nonadhesiveness of PEG hydrogel towards proteins, proteins were selectively immobilized onto surface-modified mesoporous TiO2 region, creating protein microarray. Specific binding assay between streptavidin/biotin and between PSA/anti-PSA demonstrated that the mesoporous TiO2-based protein microarrays yielded higher fluorescence signals and were more sensitive with lower detection limits than microarrays based on planar glass slides.",
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