Graphene-Iodine Nanocomposites: Highly Potent Bacterial Inhibitors that are Bio-compatible with Human Cells

Surajit Some, Ji Soo Sohn, Junmoo Kim, Su Hyun Lee, Su Chan Lee, Jungpyo Lee, Iman Shackery, Sang Kyum Kim, So Hyun Kim, Nakwon Choi, Il Joo Cho, Hyo il Jung, Shinill Kang, Seong Chan Jun

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Graphene-composites, capable of inhibiting bacterial growth which is also bio-compatible with human cells have been highly sought after. Here we report for the first time the preparation of new graphene-iodine nano-composites via electrostatic interactions between positively charged graphene derivatives and triiodide anions. The resulting composites were characterized by X-ray photoemission spectroscopy, UV-spectroscopy, Raman spectroscopy and Scanning electron microscopy. The antibacterial potential of these graphene-iodine composites against Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirobilis, Staphylococcus aureus, and E. coli was investigated. In addition, the cytotoxicity of the nanocomposite with human cells [human white blood cells (WBC), HeLa, MDA-MB-231, Fibroblast (primary human keratinocyte) and Keratinocyte (immortalized fibroblast)], was assessed. DGO (Double-oxidizes graphene oxide) was prepared by the additional oxidation of GO (graphene oxide). This generates more oxygen containing functional groups that can readily trap more H+, thus generating a positively charged surface area under highly acidic conditions. This step allowed bonding with a greater number of anionic triiodides and generated the most potent antibacterial agent among graphene-iodine and as-made povidone-iodine (PVP-I) composites also exhibited nontoxic to human cells culture. Thus, these nano-composites can be used to inhibit the growth of various bacterial species. Importantly, they are also very low-cytotoxic to human cells culture.

Original languageEnglish
Article number20015
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Feb 4

Fingerprint

Nanocomposites
Graphite
Iodine
Povidone-Iodine
Keratinocytes
Oxides
Cell Culture Techniques
Fibroblasts
Photoelectron Spectroscopy
Proteus
Raman Spectrum Analysis
Klebsiella pneumoniae
Growth
Static Electricity
Electron Scanning Microscopy
Pseudomonas aeruginosa
Anions
Staphylococcus aureus
Spectrum Analysis
Leukocytes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Some, Surajit ; Sohn, Ji Soo ; Kim, Junmoo ; Lee, Su Hyun ; Lee, Su Chan ; Lee, Jungpyo ; Shackery, Iman ; Kim, Sang Kyum ; Kim, So Hyun ; Choi, Nakwon ; Cho, Il Joo ; Jung, Hyo il ; Kang, Shinill ; Jun, Seong Chan. / Graphene-Iodine Nanocomposites : Highly Potent Bacterial Inhibitors that are Bio-compatible with Human Cells. In: Scientific reports. 2016 ; Vol. 6.
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abstract = "Graphene-composites, capable of inhibiting bacterial growth which is also bio-compatible with human cells have been highly sought after. Here we report for the first time the preparation of new graphene-iodine nano-composites via electrostatic interactions between positively charged graphene derivatives and triiodide anions. The resulting composites were characterized by X-ray photoemission spectroscopy, UV-spectroscopy, Raman spectroscopy and Scanning electron microscopy. The antibacterial potential of these graphene-iodine composites against Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirobilis, Staphylococcus aureus, and E. coli was investigated. In addition, the cytotoxicity of the nanocomposite with human cells [human white blood cells (WBC), HeLa, MDA-MB-231, Fibroblast (primary human keratinocyte) and Keratinocyte (immortalized fibroblast)], was assessed. DGO (Double-oxidizes graphene oxide) was prepared by the additional oxidation of GO (graphene oxide). This generates more oxygen containing functional groups that can readily trap more H+, thus generating a positively charged surface area under highly acidic conditions. This step allowed bonding with a greater number of anionic triiodides and generated the most potent antibacterial agent among graphene-iodine and as-made povidone-iodine (PVP-I) composites also exhibited nontoxic to human cells culture. Thus, these nano-composites can be used to inhibit the growth of various bacterial species. Importantly, they are also very low-cytotoxic to human cells culture.",
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Some, S, Sohn, JS, Kim, J, Lee, SH, Lee, SC, Lee, J, Shackery, I, Kim, SK, Kim, SH, Choi, N, Cho, IJ, Jung, HI, Kang, S & Jun, SC 2016, 'Graphene-Iodine Nanocomposites: Highly Potent Bacterial Inhibitors that are Bio-compatible with Human Cells', Scientific reports, vol. 6, 20015. https://doi.org/10.1038/srep20015

Graphene-Iodine Nanocomposites : Highly Potent Bacterial Inhibitors that are Bio-compatible with Human Cells. / Some, Surajit; Sohn, Ji Soo; Kim, Junmoo; Lee, Su Hyun; Lee, Su Chan; Lee, Jungpyo; Shackery, Iman; Kim, Sang Kyum; Kim, So Hyun; Choi, Nakwon; Cho, Il Joo; Jung, Hyo il; Kang, Shinill; Jun, Seong Chan.

In: Scientific reports, Vol. 6, 20015, 04.02.2016.

Research output: Contribution to journalArticle

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AU - Some, Surajit

AU - Sohn, Ji Soo

AU - Kim, Junmoo

AU - Lee, Su Hyun

AU - Lee, Su Chan

AU - Lee, Jungpyo

AU - Shackery, Iman

AU - Kim, Sang Kyum

AU - Kim, So Hyun

AU - Choi, Nakwon

AU - Cho, Il Joo

AU - Jung, Hyo il

AU - Kang, Shinill

AU - Jun, Seong Chan

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