Graphene multilayers as gates for multi-week sequential release of proteins from surfaces

Jinkee Hong, Nisarg J. Shah, Adam C. Drake, Peter C. Demuth, Jong Bum Lee, Jianzhu Chen, Paula T. Hammond

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The ability to control the timing and order of release of different therapeutic drugs will play a pivotal role in improving patient care and simplifying treatment regimes in the clinic. The controlled sequential release of a broad range of small and macromolecules from thin film coatings offers a simple way to provide complex localized dosing in vivo. Here we show that it is possible to take advantage of the structure of certain nanomaterials to control release regimes from a scale of hours to months. Graphene oxide (GO) is a two-dimensional charged nanomaterial that can be used to create barrier layers in multilayer thin films, trapping molecules of interest for controlled release. Protein-loaded polyelectrolyte multilayer films were fabricated using layer-by-layer assembly incorporating a hydrolytically degradable cationic poly(β-amino ester) (Poly1) with a model protein antigen, ovalbumin (ova), in a bilayer architecture along with positively and negatively functionalized GO capping layers for the degradable protein films. Ova release without the GO layers takes place in less than 1 h but can be tuned to release from 30 to 90 days by varying the number of bilayers of functionalized GO in the multilayer architecture. We demonstrate that proteins can be released in sequence with multi-day gaps between the release of each species by incorporating GO layers between protein loaded layers. In vitro toxicity assays of the individual materials on proliferating hematopoietic stem cells (HSCs) indicated limited cytotoxic effects with HSCs able to survive for the full 10 days of normal culture in the presence of Poly1 and the GO sheets. This approach provides a new route for storage of therapeutics in a solid-state thin film for subsequent delivery in a time-controlled and sequential fashion.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalACS Nano
Volume6
Issue number1
DOIs
Publication statusPublished - 2012 Jan 24

Fingerprint

Graphite
Oxides
Graphene
graphene
Multilayers
Membrane Proteins
proteins
Proteins
oxides
stem cells
Multilayer films
Stem cells
Nanostructured materials
Thin films
thin films
Ovalbumin
antigens
Antigens
barrier layers
Polyelectrolytes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Hong, J., Shah, N. J., Drake, A. C., Demuth, P. C., Lee, J. B., Chen, J., & Hammond, P. T. (2012). Graphene multilayers as gates for multi-week sequential release of proteins from surfaces. ACS Nano, 6(1), 81-88. https://doi.org/10.1021/nn202607r
Hong, Jinkee ; Shah, Nisarg J. ; Drake, Adam C. ; Demuth, Peter C. ; Lee, Jong Bum ; Chen, Jianzhu ; Hammond, Paula T. / Graphene multilayers as gates for multi-week sequential release of proteins from surfaces. In: ACS Nano. 2012 ; Vol. 6, No. 1. pp. 81-88.
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Hong, J, Shah, NJ, Drake, AC, Demuth, PC, Lee, JB, Chen, J & Hammond, PT 2012, 'Graphene multilayers as gates for multi-week sequential release of proteins from surfaces', ACS Nano, vol. 6, no. 1, pp. 81-88. https://doi.org/10.1021/nn202607r

Graphene multilayers as gates for multi-week sequential release of proteins from surfaces. / Hong, Jinkee; Shah, Nisarg J.; Drake, Adam C.; Demuth, Peter C.; Lee, Jong Bum; Chen, Jianzhu; Hammond, Paula T.

In: ACS Nano, Vol. 6, No. 1, 24.01.2012, p. 81-88.

Research output: Contribution to journalArticle

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