In this report, we have investigated enhanced surface plasmon resonance (SPR) detection of DNA hybridization using gold core - silica shell nanoparticles in localized plasmonic fields. The plasmonic fields were localized by periodic linear gratings. Experimental results measured for hybridization of 24-mer single-stranded DNA oligomers suggest that core-shell nanoparticles (CSNPs) on gratings of 400nm period provide enhanced optical signatures by 36 times over conventional thin film-based SPR detection. CSNP-mediated DNA hybridization produced 3 times larger angular shift compared to gold nanoparticles of the same core size. We have also analyzed the effect of structural variation. The enhancement using CSNPs was associated with increased surface area and index contrast that is combined by improved plasmon coupling with localized fields on gratings. The combined approach for conjugated measurement of a biomolecular interaction on grating structures is expected to lower the limit of detection to the order of a few tens of fg/mm 2.
Bibliographical noteFunding Information:
We thank Hyun Jin Min and Jeon-Soo Shin for helping control measurements. This work was supported by the National Research Foundation (NRF) grants funded by the Korean Government ( NRF-331-2008-1-D00389 , 2009-0070732 , and 2010-0007993 ).
All Science Journal Classification (ASJC) codes
- Biomedical Engineering