Green tea catechin-inactivated viral vaccine platform

Yun H. Lee, Yo H. Jang, Young H. Byun, Yucheol Cheong, Paul Kim, Young J. Lee, Yoon J. Lee, Je M. Sung, Ahyun Son, Hye M. Lee, Jinhee Lee, Seung W. Yang, Jae Min Song, Baik Lin Seong

Research output: Contribution to journalArticle

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Abstract

Traditionally, chemical agents such as formalin (FA) and β-propiolactone (BPL) have long been used for the preparation of inactivated vaccines or toxoids. It has been shown that FA extensively modifies vaccine antigens and thus affects immunogenicity profiles, sometimes compromising the protective efficacy of the vaccines or even exacerbating the disease upon infection. In this study, we show that natural catechins from green tea extracts (GT) can be used as an inactivating agent to prepare inactivated viral vaccines. GT treatment resulted in complete and irreversible inactivation of influenza virus as well as dengue virus. In contrast to FA that reacted extensively with multiple amino acids including lysine, a major anchor residue for epitope binding to MHC molecules, GT catechin epigallocatechin-3-gallate (EGCG) crosslinked primarily with cysteine residues and thus preserved the major epitopes of the influenza hemagglutinin. In a mouse model, vaccination with GT-inactivated influenza virus (GTi virus) elicited higher levels of viral neutralizing antibodies than FA-inactivated virus (FAi virus). The vaccination completely protected the mice from a lethal challenge and restricted the challenge viral replication in the lungs. Of note, the quality of antibody responses of GTi virus was superior to that with FAi virus, in terms of the magnitude of antibody titer, cross-reactivity to hetero-subtypes of influenza viruses, and the avidity to viral antigens. As the first report of using non-toxic natural compounds for the preparation of inactivated viral vaccines, the present results could be translated into a clinically relevant vaccine platform with improved efficacy, safety, productivity, and public acceptance.

Original languageEnglish
Article number2469
JournalFrontiers in Microbiology
Volume8
Issue numberDEC
DOIs
Publication statusPublished - 2017 Dec 12

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Viral Vaccines
Inactivated Vaccines
Catechin
Tea
Orthomyxoviridae
Formaldehyde
Vaccines
Epitopes
Vaccination
Propiolactone
Viral Antibodies
Toxoids
Viruses
Dengue Virus
Viral Antigens
Hemagglutinins
Neutralizing Antibodies
Human Influenza
Lysine
Antibody Formation

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Lee, Y. H., Jang, Y. H., Byun, Y. H., Cheong, Y., Kim, P., Lee, Y. J., ... Seong, B. L. (2017). Green tea catechin-inactivated viral vaccine platform. Frontiers in Microbiology, 8(DEC), [2469]. https://doi.org/10.3389/fmicb.2017.02469
Lee, Yun H. ; Jang, Yo H. ; Byun, Young H. ; Cheong, Yucheol ; Kim, Paul ; Lee, Young J. ; Lee, Yoon J. ; Sung, Je M. ; Son, Ahyun ; Lee, Hye M. ; Lee, Jinhee ; Yang, Seung W. ; Song, Jae Min ; Seong, Baik Lin. / Green tea catechin-inactivated viral vaccine platform. In: Frontiers in Microbiology. 2017 ; Vol. 8, No. DEC.
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Lee, YH, Jang, YH, Byun, YH, Cheong, Y, Kim, P, Lee, YJ, Lee, YJ, Sung, JM, Son, A, Lee, HM, Lee, J, Yang, SW, Song, JM & Seong, BL 2017, 'Green tea catechin-inactivated viral vaccine platform', Frontiers in Microbiology, vol. 8, no. DEC, 2469. https://doi.org/10.3389/fmicb.2017.02469

Green tea catechin-inactivated viral vaccine platform. / Lee, Yun H.; Jang, Yo H.; Byun, Young H.; Cheong, Yucheol; Kim, Paul; Lee, Young J.; Lee, Yoon J.; Sung, Je M.; Son, Ahyun; Lee, Hye M.; Lee, Jinhee; Yang, Seung W.; Song, Jae Min; Seong, Baik Lin.

In: Frontiers in Microbiology, Vol. 8, No. DEC, 2469, 12.12.2017.

Research output: Contribution to journalArticle

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AU - Byun, Young H.

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AU - Lee, Young J.

AU - Lee, Yoon J.

AU - Sung, Je M.

AU - Son, Ahyun

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AU - Lee, Jinhee

AU - Yang, Seung W.

AU - Song, Jae Min

AU - Seong, Baik Lin

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Lee YH, Jang YH, Byun YH, Cheong Y, Kim P, Lee YJ et al. Green tea catechin-inactivated viral vaccine platform. Frontiers in Microbiology. 2017 Dec 12;8(DEC). 2469. https://doi.org/10.3389/fmicb.2017.02469