Green tea (-)-epigallocatechin-3-gallate inhibits HGF-induced progression in oral cavity cancer through suppression of HGF/c-Met

Yoonwoo Koh, Eun Chang Choi, Sung Un Kang, Hye Sook Hwang, Mi Hye Lee, Jung Hee Pyun, Rae Hee Park, Young Don Lee, Chul Ho Kim

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Hepatocyte growth factor (HGF) and c-Met have recently attracted a great deal of attention as prognostic indicators of patient outcome, and they are important in the control of tumor growth and invasion. Epigallocatechin-3-gallate (EGCG) has been shown to modulate multiple signal pathways in a manner that controls the unwanted proliferation and invasion of cells, thereby imparting cancer chemopreventive and therapeutic effects. In this study, we investigated the effects of EGCG in inhibiting HGF-induced tumor growth and invasion of oral cancer in vitro and in vivo. We examined the effects of EGCG on HGF-induced cell proliferation, migration, invasion, induction of apoptosis and modulation of HGF/c-Met signaling pathway in the KB oral cancer cell line. We investigated the antitumor effect and inhibition of c-Met expression by EGCG in a syngeneic mouse model (C3H/HeJ mice, SCC VII/SF cell line). HGF promoted cell proliferation, migration, invasion and induction of MMP (matrix metalloproteinase)-2 and MMP-9 in KB cells. EGCG significantly inhibited HGF-induced phosphorylation of Met and cell growth, invasion and expression of MMP-2 and MMP-9. EGCG blocked HGF-induced phosphorylation of c-Met and that of the downstream kinases AKT and ERK, and inhibition of p-AKT and p-ERK by EGCG was associated with marked increases in the phosphorylation of p38, JNK, cleaved caspase-3 and poly-ADP-ribose polymerase. In C3H/HeJ syngeneic mice, as an in vivo model, tumor growth was suppressed and apoptosis was increased by EGCG. Our results suggest that EGCG may be a potential therapeutic agent to inhibit HGF-induced tumor growth and invasion in oral cancer.

Original languageEnglish
Pages (from-to)1074-1083
Number of pages10
JournalJournal of Nutritional Biochemistry
Volume22
Issue number11
DOIs
Publication statusPublished - 2011 Nov 1

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Hepatocyte Growth Factor
Mouth Neoplasms
Tea
Mouth
Phosphorylation
Tumors
Growth
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Cell Proliferation
Cell proliferation
Neoplasms
Cell Movement
Cells
Apoptosis
KB Cells
epigallocatechin gallate
Cell Line
Poly(ADP-ribose) Polymerases
Inbred C3H Mouse

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Koh, Yoonwoo ; Choi, Eun Chang ; Kang, Sung Un ; Hwang, Hye Sook ; Lee, Mi Hye ; Pyun, Jung Hee ; Park, Rae Hee ; Lee, Young Don ; Kim, Chul Ho. / Green tea (-)-epigallocatechin-3-gallate inhibits HGF-induced progression in oral cavity cancer through suppression of HGF/c-Met. In: Journal of Nutritional Biochemistry. 2011 ; Vol. 22, No. 11. pp. 1074-1083.
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Green tea (-)-epigallocatechin-3-gallate inhibits HGF-induced progression in oral cavity cancer through suppression of HGF/c-Met. / Koh, Yoonwoo; Choi, Eun Chang; Kang, Sung Un; Hwang, Hye Sook; Lee, Mi Hye; Pyun, Jung Hee; Park, Rae Hee; Lee, Young Don; Kim, Chul Ho.

In: Journal of Nutritional Biochemistry, Vol. 22, No. 11, 01.11.2011, p. 1074-1083.

Research output: Contribution to journalArticle

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T1 - Green tea (-)-epigallocatechin-3-gallate inhibits HGF-induced progression in oral cavity cancer through suppression of HGF/c-Met

AU - Koh, Yoonwoo

AU - Choi, Eun Chang

AU - Kang, Sung Un

AU - Hwang, Hye Sook

AU - Lee, Mi Hye

AU - Pyun, Jung Hee

AU - Park, Rae Hee

AU - Lee, Young Don

AU - Kim, Chul Ho

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