Growth cones contain myosin II bipolar filament arrays

Mi Ra Suh, Ji Woong Han, Yi Ran No, JooHun Lee

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Nonmuscle myosin II is among the most abundant forms of myosin in nerve growth cones. At least two isoforms of myosin II (A and B) that have overlapping but distinct distributions are found in growth cones. It appears that both myosin IIA and IIB may be necessary for normal nerve outgrowth and motility, but the molecular interactions responsible for their activity remain unclear. For instance, it is unknown if these myosin II isoforms produce bipolar "minifilaments" in growth cones similar to those observed in other nonmuscle cells. To determine if minifilaments are present in growth cones, we modified the electron microscopy preparative procedures used to detect minifilaments in other cell types. We found structures that appeared very similar to bipolar minifilaments found in noneuronal cells. They also labeled with antibodies to either myosin IIA or IIB. Thus, the activity of myosin II in growth cones is likely to be similar to that in other nonmuscle cells. Bipolar filaments interacting with oppositely oriented actin filaments will produce localized contractions or exert tension on actin networks. This activity will be responsible for the myosin II dependent motility in growth cones.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalCell Motility and the Cytoskeleton
Volume52
Issue number2
DOIs
Publication statusPublished - 2002 Jun 8

Fingerprint

Myosin Type II
Growth Cones
Nonmuscle Myosin Type IIB
Nonmuscle Myosin Type IIA
Protein Isoforms
Myosins
Actin Cytoskeleton
Actins
Electron Microscopy
Antibodies

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Cell Biology

Cite this

Suh, Mi Ra ; Han, Ji Woong ; No, Yi Ran ; Lee, JooHun. / Growth cones contain myosin II bipolar filament arrays. In: Cell Motility and the Cytoskeleton. 2002 ; Vol. 52, No. 2. pp. 91-96.
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Growth cones contain myosin II bipolar filament arrays. / Suh, Mi Ra; Han, Ji Woong; No, Yi Ran; Lee, JooHun.

In: Cell Motility and the Cytoskeleton, Vol. 52, No. 2, 08.06.2002, p. 91-96.

Research output: Contribution to journalArticle

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