Growth of mycobacteria on carbon monoxide and methanol

Sae W. Park, Eun H. Hwang, Hyuck Park, Jeong A. Kim, Jinho Heo, Key H. Lee, Taeksun Song, Eungbin KIm, Young T. Ro, Si W. Kim, Young M. Kim

Research output: Contribution to journalArticle

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Abstract

Several mycobacterial strains, such as Mycobacterium flavescens, Mycobacterium gastri, Mycobacterium neoaurum, Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium vaccae, were found to grow on carbon monoxide (CO) as the sole source of carbon and energy. These bacteria, except for M. tuberculosis, also utilized methanol as the sole carbon and energy source. A CO dehydrogenase (CO-DH) assay, staining by activity of CO-DH, and Western blot analysis using an antibody raised against CO-DH of Mycobacterium sp. strain JC1 (formerly Acinetobacter sp. strain JC1 [J. W. Cho, H. S. Yim, and Y. M. Kim, Kor. J. Microbiol. 23:1-8, 1985]) revealed that CO-DH is present in extracts of the bacteria prepared from cells grown on CO. Ribulose bisphosphate carboxylase/oxygenase (RubisCO) activity was also detected in extracts prepared from all cells, except M. tuberculosis, grown on CO. The mycobacteria grown on methanol, except for M. gastri, which showed hexulose phosphate synthase activity, did not exhibit activities of classic methanol dehydrogenase, hydroxypyruvate reductase, or hexulose phosphate synthase but exhibited N,N-dimethyl-4-nitrosoaniline-dependent methanol dehydrogenase and RuBisCO activities. Cells grown on methanol were also found to have dihydroxyacetone synthase. Double immunodiffusion revealed that the antigenic sites of CO-DHs, RuBisCOs, and dihydroxyacetone synthases in all mycobacteria tested are identical with those of the Mycobacterium sp. strain JC1 enzymes.

Original languageEnglish
Pages (from-to)142-147
Number of pages6
JournalJournal of Bacteriology
Volume185
Issue number1
DOIs
Publication statusPublished - 2003 Jan 1

Fingerprint

Carbon Monoxide
Mycobacterium
Methanol
formaldehyde transketolase
Growth
Mycobacterium tuberculosis
carbon monoxide dehydrogenase
Hydroxypyruvate Reductase
Carbon
Mycobacterium phlei
Phosphates
Ribulose-Bisphosphate Carboxylase
Mycobacterium smegmatis
Bacteria
Nontuberculous Mycobacteria
Oxygenases
Acinetobacter
Immunodiffusion
Western Blotting
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Park, S. W., Hwang, E. H., Park, H., Kim, J. A., Heo, J., Lee, K. H., ... Kim, Y. M. (2003). Growth of mycobacteria on carbon monoxide and methanol. Journal of Bacteriology, 185(1), 142-147. https://doi.org/10.1128/JB.185.1.142-147.2003
Park, Sae W. ; Hwang, Eun H. ; Park, Hyuck ; Kim, Jeong A. ; Heo, Jinho ; Lee, Key H. ; Song, Taeksun ; KIm, Eungbin ; Ro, Young T. ; Kim, Si W. ; Kim, Young M. / Growth of mycobacteria on carbon monoxide and methanol. In: Journal of Bacteriology. 2003 ; Vol. 185, No. 1. pp. 142-147.
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Park, SW, Hwang, EH, Park, H, Kim, JA, Heo, J, Lee, KH, Song, T, KIm, E, Ro, YT, Kim, SW & Kim, YM 2003, 'Growth of mycobacteria on carbon monoxide and methanol', Journal of Bacteriology, vol. 185, no. 1, pp. 142-147. https://doi.org/10.1128/JB.185.1.142-147.2003

Growth of mycobacteria on carbon monoxide and methanol. / Park, Sae W.; Hwang, Eun H.; Park, Hyuck; Kim, Jeong A.; Heo, Jinho; Lee, Key H.; Song, Taeksun; KIm, Eungbin; Ro, Young T.; Kim, Si W.; Kim, Young M.

In: Journal of Bacteriology, Vol. 185, No. 1, 01.01.2003, p. 142-147.

Research output: Contribution to journalArticle

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Park SW, Hwang EH, Park H, Kim JA, Heo J, Lee KH et al. Growth of mycobacteria on carbon monoxide and methanol. Journal of Bacteriology. 2003 Jan 1;185(1):142-147. https://doi.org/10.1128/JB.185.1.142-147.2003