Growth on methanol of a carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803

Young Tae Ro, Jae Goo Seo, JooHun Lee, Daemyung Kim, In Kwon Chung, Tae Ue Kim, Young Min Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, was found to grow methylotrophically at the expense of methanol and methylamine, but not of methane, formaldehyde, formate, dimethylamine, or trimethylamine, as the sole source of carbon and energy. The doubling times of the bacterium growing on methanol (0.5%, v/v) and methylamine (0.5%, w/v) at 30°C and pH 6.8 were 4.8 h and 5.7 h, respectively. Cells grown on methanol, however, failed to show typical methanol dehydrogenase and oxiclase activities. The cell was found to contain no c-type cytochromes. Cells grown on methanol exhibited higher catalase activity than those grown on pyruvate or glucose. The catalase present in the cells also exhibited peroxidase activity. The catalase activity, growth on methanol of the cell, and oxygen consumption by methanol-grown cells were inhibited strongly by 0.1 mM, 1 mM, and 0.1 mM hydroxylamine, respectively. Formaldehyde dehydrogenase, formaldehyde reductase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase activities were detected from cells grown on methanol.

Original languageEnglish
Pages (from-to)30-39
Number of pages10
JournalJournal of Microbiology
Volume35
Issue number1
Publication statusPublished - 1997 Mar 1

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Acinetobacter
Methanol
Growth
Catalase
formic acid
glutathione-independent formaldehyde dehydrogenase
Cytochrome c Group
Phosphogluconate Dehydrogenase
Hydroxylamine
Glucosephosphate Dehydrogenase
Methane
Pyruvic Acid
Oxygen Consumption
Formaldehyde
Peroxidase
Carbon
Bacteria
Glucose

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Ro, Young Tae ; Seo, Jae Goo ; Lee, JooHun ; Kim, Daemyung ; Chung, In Kwon ; Kim, Tae Ue ; Kim, Young Min. / Growth on methanol of a carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803. In: Journal of Microbiology. 1997 ; Vol. 35, No. 1. pp. 30-39.
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abstract = "Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, was found to grow methylotrophically at the expense of methanol and methylamine, but not of methane, formaldehyde, formate, dimethylamine, or trimethylamine, as the sole source of carbon and energy. The doubling times of the bacterium growing on methanol (0.5{\%}, v/v) and methylamine (0.5{\%}, w/v) at 30°C and pH 6.8 were 4.8 h and 5.7 h, respectively. Cells grown on methanol, however, failed to show typical methanol dehydrogenase and oxiclase activities. The cell was found to contain no c-type cytochromes. Cells grown on methanol exhibited higher catalase activity than those grown on pyruvate or glucose. The catalase present in the cells also exhibited peroxidase activity. The catalase activity, growth on methanol of the cell, and oxygen consumption by methanol-grown cells were inhibited strongly by 0.1 mM, 1 mM, and 0.1 mM hydroxylamine, respectively. Formaldehyde dehydrogenase, formaldehyde reductase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase activities were detected from cells grown on methanol.",
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Growth on methanol of a carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803. / Ro, Young Tae; Seo, Jae Goo; Lee, JooHun; Kim, Daemyung; Chung, In Kwon; Kim, Tae Ue; Kim, Young Min.

In: Journal of Microbiology, Vol. 35, No. 1, 01.03.1997, p. 30-39.

Research output: Contribution to journalArticle

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T1 - Growth on methanol of a carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803

AU - Ro, Young Tae

AU - Seo, Jae Goo

AU - Lee, JooHun

AU - Kim, Daemyung

AU - Chung, In Kwon

AU - Kim, Tae Ue

AU - Kim, Young Min

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N2 - Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, was found to grow methylotrophically at the expense of methanol and methylamine, but not of methane, formaldehyde, formate, dimethylamine, or trimethylamine, as the sole source of carbon and energy. The doubling times of the bacterium growing on methanol (0.5%, v/v) and methylamine (0.5%, w/v) at 30°C and pH 6.8 were 4.8 h and 5.7 h, respectively. Cells grown on methanol, however, failed to show typical methanol dehydrogenase and oxiclase activities. The cell was found to contain no c-type cytochromes. Cells grown on methanol exhibited higher catalase activity than those grown on pyruvate or glucose. The catalase present in the cells also exhibited peroxidase activity. The catalase activity, growth on methanol of the cell, and oxygen consumption by methanol-grown cells were inhibited strongly by 0.1 mM, 1 mM, and 0.1 mM hydroxylamine, respectively. Formaldehyde dehydrogenase, formaldehyde reductase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase activities were detected from cells grown on methanol.

AB - Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, was found to grow methylotrophically at the expense of methanol and methylamine, but not of methane, formaldehyde, formate, dimethylamine, or trimethylamine, as the sole source of carbon and energy. The doubling times of the bacterium growing on methanol (0.5%, v/v) and methylamine (0.5%, w/v) at 30°C and pH 6.8 were 4.8 h and 5.7 h, respectively. Cells grown on methanol, however, failed to show typical methanol dehydrogenase and oxiclase activities. The cell was found to contain no c-type cytochromes. Cells grown on methanol exhibited higher catalase activity than those grown on pyruvate or glucose. The catalase present in the cells also exhibited peroxidase activity. The catalase activity, growth on methanol of the cell, and oxygen consumption by methanol-grown cells were inhibited strongly by 0.1 mM, 1 mM, and 0.1 mM hydroxylamine, respectively. Formaldehyde dehydrogenase, formaldehyde reductase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase activities were detected from cells grown on methanol.

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