GT1b ganglioside induces death of dopaminergic neurons in rat mesencephalic cultures

Eun S. Chung, Eun H. Joe, Jae K. Ryu, Jean Kim, Yong B. Lee, Kyung G. Cho, Young J. Oh, Seung H. Maeng, Hyung H. Baik, Seung U. Kim, Byung K. Jin

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19 Citations (Scopus)

Abstract

We examined neurotoxicity of GT1b against dopaminergic neurons in vitro. Cultures of mesencephalic cells deprived of serum underwent the loss of 19% of tyrosine hydroxylase immunopositive (TH-ip) neurons. In cultures deprived of serum, treatment with 10-30μg/ml GT1b attenuated the number of TH-ip neurons by 26-69%, respectively, compared to non-treated cultures. Intriguingly, cultures deprived of serum were more vulnerable to GT1b-induced neurotoxicity. Application of 60μg/ml GT1b to cultures grown in serum containing media resulted in the loss of 26% of TH-ip neurons, similar to that (28%) observed in serum-deprived cultures treated with 10μ/ml GT1b. Moreover, in our cultures, absence of nitric oxide (NO) production after GT1b treatment was obvious. The present results strongly suggest direct neurotoxic actions of GT1b against dopaminergic neurons regardless of NO.

Original languageEnglish
Pages (from-to)611-614
Number of pages4
JournalNeuroReport
Volume12
Issue number3
DOIs
Publication statusPublished - 2001 Mar 5

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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    Chung, E. S., Joe, E. H., Ryu, J. K., Kim, J., Lee, Y. B., Cho, K. G., Oh, Y. J., Maeng, S. H., Baik, H. H., Kim, S. U., & Jin, B. K. (2001). GT1b ganglioside induces death of dopaminergic neurons in rat mesencephalic cultures. NeuroReport, 12(3), 611-614. https://doi.org/10.1097/00001756-200103050-00036