Hair growth-promoting effect of Aconiti Ciliare Tuber extract mediated by the activation of Wnt/β-catenin signaling

Phil June Park, Byoung San Moon, Soung Hoon Lee, Su Na Kim, Ah Reum Kim, Hyung Jun Kim, Won Seok Park, Kang Yell Choi, Eun Gyung Cho, Tae Ryong Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Aims: The activation of Wnt/β-catenin signaling pathway plays an important role in hair follicle morphogenesis by stimulating bulge stem cells. This study was to obtain the activator of Wnt/β-catenin signaling pathway from natural products and to determine whether this activator can induce anagen hair growth in mice. Main methods: To identify materials that activate Wnt/β-catenin signaling pathway, 800 natural product extracts were screened using pTOPFlash assay and neural progenitor cell (NPC) differentiation assay. A selected extract was further tested for its effects on alkaline phosphatase (ALP) activity in human immortalized dermal papilla cell (iDPC) and the proliferation in iDPC and immortalized rat vibrissa DPC (RvDP). Finally, hair growth-promoting effects were evaluated in the dorsal skin of C57BL/6 mice. Key findings: Aconiti Ciliare Tuber (ACT) extract was one of the most active materials in both pTOPFlash and NPC differentiation assays. It promoted the differentiation of NPC cells even under proliferation-stimulating conditions (basic fibroblast growth factor: bFGF). It also increased ALP activity and proliferation of iDPC in dose-dependent manners, and it stimulated the induction of the anagen hair growth in C57BL/6 mice. These results suggest that ACT extract activates the Wnt/β-catenin signaling pathway by enhancing β-catenin transcription and has the potential to promote the induction of hair growth via activation of the stem cell activity of the dermal papilla cells. Significance: This is the first report indicating benefits of ACT extract in hair loss prevention by triggering the activation of Wnt/β-catenin signaling pathway and induction of the anagen hair growth in mice.

Original languageEnglish
Pages (from-to)935-943
Number of pages9
JournalLife Sciences
Volume91
Issue number19-20
DOIs
Publication statusPublished - 2012 Nov 2

Fingerprint

Catenins
Wnt Signaling Pathway
Hair
Chemical activation
Stem Cells
Growth
Skin
Assays
Stem cells
Biological Products
Inbred C57BL Mouse
Alkaline Phosphatase
Cell Differentiation
Loss prevention
Vibrissae
Hair Follicle
Alopecia
Fibroblast Growth Factor 2
Transcription
Morphogenesis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Park, P. J., Moon, B. S., Lee, S. H., Kim, S. N., Kim, A. R., Kim, H. J., ... Lee, T. R. (2012). Hair growth-promoting effect of Aconiti Ciliare Tuber extract mediated by the activation of Wnt/β-catenin signaling. Life Sciences, 91(19-20), 935-943. https://doi.org/10.1016/j.lfs.2012.09.008
Park, Phil June ; Moon, Byoung San ; Lee, Soung Hoon ; Kim, Su Na ; Kim, Ah Reum ; Kim, Hyung Jun ; Park, Won Seok ; Choi, Kang Yell ; Cho, Eun Gyung ; Lee, Tae Ryong. / Hair growth-promoting effect of Aconiti Ciliare Tuber extract mediated by the activation of Wnt/β-catenin signaling. In: Life Sciences. 2012 ; Vol. 91, No. 19-20. pp. 935-943.
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abstract = "Aims: The activation of Wnt/β-catenin signaling pathway plays an important role in hair follicle morphogenesis by stimulating bulge stem cells. This study was to obtain the activator of Wnt/β-catenin signaling pathway from natural products and to determine whether this activator can induce anagen hair growth in mice. Main methods: To identify materials that activate Wnt/β-catenin signaling pathway, 800 natural product extracts were screened using pTOPFlash assay and neural progenitor cell (NPC) differentiation assay. A selected extract was further tested for its effects on alkaline phosphatase (ALP) activity in human immortalized dermal papilla cell (iDPC) and the proliferation in iDPC and immortalized rat vibrissa DPC (RvDP). Finally, hair growth-promoting effects were evaluated in the dorsal skin of C57BL/6 mice. Key findings: Aconiti Ciliare Tuber (ACT) extract was one of the most active materials in both pTOPFlash and NPC differentiation assays. It promoted the differentiation of NPC cells even under proliferation-stimulating conditions (basic fibroblast growth factor: bFGF). It also increased ALP activity and proliferation of iDPC in dose-dependent manners, and it stimulated the induction of the anagen hair growth in C57BL/6 mice. These results suggest that ACT extract activates the Wnt/β-catenin signaling pathway by enhancing β-catenin transcription and has the potential to promote the induction of hair growth via activation of the stem cell activity of the dermal papilla cells. Significance: This is the first report indicating benefits of ACT extract in hair loss prevention by triggering the activation of Wnt/β-catenin signaling pathway and induction of the anagen hair growth in mice.",
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Park, PJ, Moon, BS, Lee, SH, Kim, SN, Kim, AR, Kim, HJ, Park, WS, Choi, KY, Cho, EG & Lee, TR 2012, 'Hair growth-promoting effect of Aconiti Ciliare Tuber extract mediated by the activation of Wnt/β-catenin signaling', Life Sciences, vol. 91, no. 19-20, pp. 935-943. https://doi.org/10.1016/j.lfs.2012.09.008

Hair growth-promoting effect of Aconiti Ciliare Tuber extract mediated by the activation of Wnt/β-catenin signaling. / Park, Phil June; Moon, Byoung San; Lee, Soung Hoon; Kim, Su Na; Kim, Ah Reum; Kim, Hyung Jun; Park, Won Seok; Choi, Kang Yell; Cho, Eun Gyung; Lee, Tae Ryong.

In: Life Sciences, Vol. 91, No. 19-20, 02.11.2012, p. 935-943.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hair growth-promoting effect of Aconiti Ciliare Tuber extract mediated by the activation of Wnt/β-catenin signaling

AU - Park, Phil June

AU - Moon, Byoung San

AU - Lee, Soung Hoon

AU - Kim, Su Na

AU - Kim, Ah Reum

AU - Kim, Hyung Jun

AU - Park, Won Seok

AU - Choi, Kang Yell

AU - Cho, Eun Gyung

AU - Lee, Tae Ryong

PY - 2012/11/2

Y1 - 2012/11/2

N2 - Aims: The activation of Wnt/β-catenin signaling pathway plays an important role in hair follicle morphogenesis by stimulating bulge stem cells. This study was to obtain the activator of Wnt/β-catenin signaling pathway from natural products and to determine whether this activator can induce anagen hair growth in mice. Main methods: To identify materials that activate Wnt/β-catenin signaling pathway, 800 natural product extracts were screened using pTOPFlash assay and neural progenitor cell (NPC) differentiation assay. A selected extract was further tested for its effects on alkaline phosphatase (ALP) activity in human immortalized dermal papilla cell (iDPC) and the proliferation in iDPC and immortalized rat vibrissa DPC (RvDP). Finally, hair growth-promoting effects were evaluated in the dorsal skin of C57BL/6 mice. Key findings: Aconiti Ciliare Tuber (ACT) extract was one of the most active materials in both pTOPFlash and NPC differentiation assays. It promoted the differentiation of NPC cells even under proliferation-stimulating conditions (basic fibroblast growth factor: bFGF). It also increased ALP activity and proliferation of iDPC in dose-dependent manners, and it stimulated the induction of the anagen hair growth in C57BL/6 mice. These results suggest that ACT extract activates the Wnt/β-catenin signaling pathway by enhancing β-catenin transcription and has the potential to promote the induction of hair growth via activation of the stem cell activity of the dermal papilla cells. Significance: This is the first report indicating benefits of ACT extract in hair loss prevention by triggering the activation of Wnt/β-catenin signaling pathway and induction of the anagen hair growth in mice.

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