Hamster diphtheria toxin receptor: A naturally occurring chimera of monkey and mouse HB-EGF precursors

JeongHeon Cha, Joanna S. Brooke, Leon Eidels

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The sensitivity of mammalian cell lines to diphtheria toxin (DT) varies between species. Monkey (Mk) Vero cells are highly sensitive to DT, whereas rat and mouse (Ms) cells are resistant; hamster (Hm) cells display moderate DT sensitivity. The precursor of the Mk heparin-binding epidermal growth factor-like growth factor (proHB-EGF) functions as a DT receptor but the Ms proHB-EGF does not. In this study we have cloned, expressed, and characterized the Hm proHB-EGF/DT receptor. The expression of Hm proHB-EGF confers moderate DT sensitivity to normally DT-resistant mouse cells. The amino acid sequence of Hm preproHB-EGF shows that, overall, it more closely resembles the Ms preproHB-EGF sequence, except in the DT-binding region where it more closely resembles the Mk sequence. In the DT-binding region the Hm proHB-EGF sequence differs from the Mk proHB-EGF in only four amino acid residues (124, 126, 133, and 147); one of these residues, Ile133 in Mk proHB-EGF, has been previously reported to be important for DT binding and sensitivity. Analysis of Mk proHB-EGF mutants with residues substituted for Ile133 suggests that Asn133 in Hm proHB-EGF may be responsible for the moderate DT sensitivity of Hm proHB-EGF-expressing cells.

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume254
Issue number2
DOIs
Publication statusPublished - 1999 Jan 19

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Diphtheria Toxin
Epidermal Growth Factor
Cricetinae
Haplorhini
Heparin
Intercellular Signaling Peptides and Proteins
Heparin-binding EGF-like Growth Factor
Cells
Amino Acids
Vero Cells
Rats
Amino Acid Sequence
Cell Line

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "Hamster diphtheria toxin receptor: A naturally occurring chimera of monkey and mouse HB-EGF precursors",
abstract = "The sensitivity of mammalian cell lines to diphtheria toxin (DT) varies between species. Monkey (Mk) Vero cells are highly sensitive to DT, whereas rat and mouse (Ms) cells are resistant; hamster (Hm) cells display moderate DT sensitivity. The precursor of the Mk heparin-binding epidermal growth factor-like growth factor (proHB-EGF) functions as a DT receptor but the Ms proHB-EGF does not. In this study we have cloned, expressed, and characterized the Hm proHB-EGF/DT receptor. The expression of Hm proHB-EGF confers moderate DT sensitivity to normally DT-resistant mouse cells. The amino acid sequence of Hm preproHB-EGF shows that, overall, it more closely resembles the Ms preproHB-EGF sequence, except in the DT-binding region where it more closely resembles the Mk sequence. In the DT-binding region the Hm proHB-EGF sequence differs from the Mk proHB-EGF in only four amino acid residues (124, 126, 133, and 147); one of these residues, Ile133 in Mk proHB-EGF, has been previously reported to be important for DT binding and sensitivity. Analysis of Mk proHB-EGF mutants with residues substituted for Ile133 suggests that Asn133 in Hm proHB-EGF may be responsible for the moderate DT sensitivity of Hm proHB-EGF-expressing cells.",
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Hamster diphtheria toxin receptor : A naturally occurring chimera of monkey and mouse HB-EGF precursors. / Cha, JeongHeon; Brooke, Joanna S.; Eidels, Leon.

In: Biochemical and Biophysical Research Communications, Vol. 254, No. 2, 19.01.1999, p. 325-329.

Research output: Contribution to journalArticle

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