Near infrared (NIR) photothermal pattern on conductive polymer film enables unique approaches to harvest large-area cell sheets with various patterns without the use of patterned culture dish. The NIR photothermal pattern is generated from a patterned optical lens (POL), which creates a dynamic near IR light pattern and the corresponding photothermal pattern (PTP) on the polymer film. The POL is prepared from transparent polydimethylsiloxane designed to generate various light patterns. The PTPs allow a noninvasive harvest of cultured cells as an intact living cell sheet with a high harvesting efficiency (ηcell > 100). Various PTPs are generated by the diffraction of NIR light through POLs having different micropatterns, which afford cell sheets with a desired pattern without changing the original cell morphology at cultured state. Furthermore, a large-area living cell sheet is obtained with a detached area larger than 19 cm2, which is the largest living cell sheet up to date. Further optical engineering of the harvesting system allows multiple productions of cell sheets with one dose of light. It is possible to harvest cell sheets not only from human fibroblast cells but also from human adipose-derived stem cells, indicating that the method can be applied to engineer various cells.
Bibliographical noteFunding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI15C0942). The authors acknowledge the financial support of the National Research Foundation (NRF) grant funded by the Korean government (Ministry of Science, ICT & Future Planning, MSIP) through the Active Polymer Center for Pattern Integration (APCPI) (2007?0056091).
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics