Heat Shock Protein, αB Crystallin, Can Prevent the Arrhythmogenic Effect of Ambient Particulate Matter by Attenuating the Repolarization Gradient and Triggered Activity

Hyelim Park, Hui Nam Pak, Moon Hyoung Lee, Ji Hyung Chung, Boyoung Joung

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Ambient particulate matter (PM) can increase cardiac arrhythmias via oxidative stress. This study was performed to reveal that the heat shock protein, α B-crystallin, could prevent the arrhythmogenic effect of PM. Methods: Using optical mapping, arrhythmic mechanism were evaluated in Langendorff-perfused Adult Sprague-Dawley rat heart after infusion of diesel exhaust product (DEP) and αB-crystallin. Results: In neonatal rat cardiomyocytes, DEP provoked cell death and ROS generation in dose dependant manner. In Langendorff-perfused rat hearts, DEP infusion of 12.5 mg/L (n=12) prolonged action potential duration (APD90) at only the base of left ventricle (LV) from 101± 14 ms to 152± 22 ms (p=0.001) increasing apicobasal APD differences from 4± 8 ms to 54± 25 ms (p=0.003). Pretreatment of aB-crystallin (1 mg/kg, n=9) with a delivery system (TAT-protein transduction domain) prevented the DEP-induced APD prolongation (106± 10 ms, p=0.83) and apicobasal APD differences (1.5± 15.5 ms, p=0.83). Compared with DEP alone, pretreatment of αB-crystallin decreased triggered activity (67% vs. 11%, p=0.02) and apicobasal reentry (82% vs. 0%, p=0.001). The ventricular tachyarrhythmia (VT) was observed in 9 (75%) and 4 (44%) hearts after DEP alone and pretreatment of αB-crystallin (p=0.04), respectively. Conclusions: Heat shock protein, αB-crystallin, can prevent arrhythmogenic effect of PM by suppressing apicobasal repolarization gradient and triggered activity.

Original languageEnglish
Journaljournal of arrhythmia
Volume27
DOIs
Publication statusPublished - 2011

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

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