Heat shock protein X purified from Mycobacterium tuberculosis enhances the efficacy of dendritic cells-based immunotherapy for the treatment of allergic asthma

Hye Young Kim, Hyun Kyu Kang, Joon Cho, In Duk Jung, Gun Young Yoon, Min Goo Lee, SungJae Shin, Won Sun Park, Jong Hwan Park, Seung Wook Ryu, Yeong Min Park, Ji Chang You

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dendritic cells play an important role in determining whether naïve T cells mature into either Th1 or Th2 cells. We determined whether heat-shock protein X (HspX) purified from Mycobacterium tuberculosis regulates the Th1/Th2 immune response in an ovalbumin (OVA)-induced murine model of asthma. HspX increased interferon-gamma, IL-17A, -12 and transforming growth factor (TGF)-β production and T-bet gene expression but reduced IL-13 production and GATA-3 gene expression. HspX also inhibited asthmatic reactions as demonstrated by an increase in the number of eosinophils in bronchoalveolar lavage fluid, inflammatory cell infiltration in lung tissues, airway luminal narrowing, and airway hyper-responsiveness. Furthermore, HspX enhanced OVA-induced decrease of regulatory T cells in the mediastinal lymph nodes. This study provides evidence that HspX plays critical roles in the amelioration of asthmatic inflammation in mice. These findings provide new insights into the immunotherapeutic role of HspX with respect to its effects on a murine model of asthma.

Original languageEnglish
Pages (from-to)178-183
Number of pages6
JournalBMB reports
Volume48
Issue number3
DOIs
Publication statusPublished - 2015 Jan 1

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Heat-Shock Proteins
Mycobacterium tuberculosis
Immunotherapy
Dendritic Cells
Asthma
T-cells
Ovalbumin
Gene expression
Respiratory Hypersensitivity
Therapeutics
Gene Expression
Th2 Cells
Th1 Cells
Interleukin-13
Interleukin-17
Bronchoalveolar Lavage Fluid
Transforming Growth Factors
Regulatory T-Lymphocytes
Interleukin-12
Infiltration

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Kim, Hye Young ; Kang, Hyun Kyu ; Cho, Joon ; Jung, In Duk ; Yoon, Gun Young ; Lee, Min Goo ; Shin, SungJae ; Park, Won Sun ; Park, Jong Hwan ; Ryu, Seung Wook ; Park, Yeong Min ; You, Ji Chang. / Heat shock protein X purified from Mycobacterium tuberculosis enhances the efficacy of dendritic cells-based immunotherapy for the treatment of allergic asthma. In: BMB reports. 2015 ; Vol. 48, No. 3. pp. 178-183.
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Heat shock protein X purified from Mycobacterium tuberculosis enhances the efficacy of dendritic cells-based immunotherapy for the treatment of allergic asthma. / Kim, Hye Young; Kang, Hyun Kyu; Cho, Joon; Jung, In Duk; Yoon, Gun Young; Lee, Min Goo; Shin, SungJae; Park, Won Sun; Park, Jong Hwan; Ryu, Seung Wook; Park, Yeong Min; You, Ji Chang.

In: BMB reports, Vol. 48, No. 3, 01.01.2015, p. 178-183.

Research output: Contribution to journalArticle

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AU - You, Ji Chang

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