Helicobacter pylori CagA transfection of gastric epithelial cells induces interleukin-8

Seok Yong Kim, Yongchan Lee, Hyong Kyu Kim, Martin J. Blaser

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

To determine the effect of Helicobacter pylori CagA expression on interleukin-8 (IL-8) induction in AGS cells, cagA and five of its fragments from strains 147A and 147C that vary in the 3′ repeat region were cloned into the eukaryotic expression plasmid pSP65SRα. IL-8, but not RANTES or IL-Iβ, levels were increased in AGS cells transfected with 147A-cagA and to a greater extent with 147C-cagA, compared with negative controls. The 5′ b fragment from the two strains had similar effects, but the 3′ d and e fragments from 147C CagA had greater effects than those from 147A-CagA. When the Western CagA-specific sequence (WSS) of 147C-cagA was replaced with East Asian CagA-specific sequence (ESS) and cloned into pSP65SRα as an East/West chimera, there was no significant effect on IL-8 production. Use of specific inhibitors indicates that Src kinase activation, and the mitogen-activated protein (MAP) kinase and NF-κB pathways are the major intermediates for CagA effects on IL-8 induction, but the p38 MAP kinase pathway has little effect. These results indicate a direct CagA effect on IL-8 induction by gastric epithelial cells, and indicate signal pathway loci that can be targeted for amelioration.

Original languageEnglish
Pages (from-to)97-106
Number of pages10
JournalCellular Microbiology
Volume8
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

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Interleukin-8
Helicobacter pylori
Transfection
Stomach
Epithelial Cells
Chemokine CCL5
Proto-Oncogene Proteins c-akt
src-Family Kinases
p38 Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
Signal Transduction
Plasmids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Kim, Seok Yong ; Lee, Yongchan ; Kim, Hyong Kyu ; Blaser, Martin J. / Helicobacter pylori CagA transfection of gastric epithelial cells induces interleukin-8. In: Cellular Microbiology. 2006 ; Vol. 8, No. 1. pp. 97-106.
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Helicobacter pylori CagA transfection of gastric epithelial cells induces interleukin-8. / Kim, Seok Yong; Lee, Yongchan; Kim, Hyong Kyu; Blaser, Martin J.

In: Cellular Microbiology, Vol. 8, No. 1, 01.01.2006, p. 97-106.

Research output: Contribution to journalArticle

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