Helicobacter pylori in a Korean isolate expressed proteins differentially in human gastric epithelial cells

Soon Ok Cho, Joo Weon Lim, Jong Ho Jun, Kyung Hwan Kim, Hyeyoung Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: The proteins expressed in gastric epithelial cells infected with Helicobacter pylori (H. pylori) may determine the clinical outcome such as chronic gastritis, peptic ulcer, and gastric carcinoma. The present study aims to determine the differentially expressed proteins in human gastric epithelial AGS cells that were infected with H. pylori in a Korean isolate, a cagA+, vacA s1b m2 iceA1 H. pylori by proteomic analysis. The differentially expressed proteins, whose expression levels were more or less than twofold in H. pylori-infected cells, were analyzed. Results: Ten proteins (chromatin assembly factor-1, proliferating cell nuclear antigen, 14-3-3 protein τ, eukaryotic translation initiation factor 6, heat-shock protein 90β, dimethylarginine dimethylaminohydrolase-1, l-lactate dehydrogenase B chain, prohibitin, triosephosphate isomerase, protein disulfide isomerase) were up-regulated while eight proteins (heat-shock gp96 precursor, nucleophosmin, ornithine aminotransferase, Ku70, l-arginine-glycine amidinotransferase, Smad anchor for receptor activation, ADP-ribosylation factor, WD repeat-containing protein isoform 1) were down-regulated by H. pylori infection in AGS cells. These proteins are related to cell proliferation, cell adhesion, carcinogenesis, cell-defense mechanisms against oxidative stress, membrane trafficking, and energy metabolism. Conclusions: Oxidative stress, cell proliferation, cell adhesion, and membrane trafficking may be involved in the pathogenesis of gastric diseases including cancer associated with H. pylori in a Korean isolate.

Original languageEnglish
Pages (from-to)1550-1564
Number of pages15
JournalDigestive diseases and sciences
Volume55
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Helicobacter pylori
Stomach
Epithelial Cells
Proteins
Cell Adhesion
Chromatin Assembly Factor-1
Oxidative Stress
Translational Peptide Chain Initiation
Ornithine-Oxo-Acid Transaminase
Cell Proliferation
ADP-Ribosylation Factors
Triose-Phosphate Isomerase
14-3-3 Proteins
Eukaryotic Initiation Factors
Protein Disulfide-Isomerases
HSP90 Heat-Shock Proteins
Stomach Diseases
Proliferating Cell Nuclear Antigen
Helicobacter Infections
Gastritis

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

Cite this

Cho, Soon Ok ; Lim, Joo Weon ; Jun, Jong Ho ; Kim, Kyung Hwan ; Kim, Hyeyoung. / Helicobacter pylori in a Korean isolate expressed proteins differentially in human gastric epithelial cells. In: Digestive diseases and sciences. 2010 ; Vol. 55, No. 6. pp. 1550-1564.
@article{b4c66c849e7944b18a1b7d93e9f81471,
title = "Helicobacter pylori in a Korean isolate expressed proteins differentially in human gastric epithelial cells",
abstract = "Purpose: The proteins expressed in gastric epithelial cells infected with Helicobacter pylori (H. pylori) may determine the clinical outcome such as chronic gastritis, peptic ulcer, and gastric carcinoma. The present study aims to determine the differentially expressed proteins in human gastric epithelial AGS cells that were infected with H. pylori in a Korean isolate, a cagA+, vacA s1b m2 iceA1 H. pylori by proteomic analysis. The differentially expressed proteins, whose expression levels were more or less than twofold in H. pylori-infected cells, were analyzed. Results: Ten proteins (chromatin assembly factor-1, proliferating cell nuclear antigen, 14-3-3 protein τ, eukaryotic translation initiation factor 6, heat-shock protein 90β, dimethylarginine dimethylaminohydrolase-1, l-lactate dehydrogenase B chain, prohibitin, triosephosphate isomerase, protein disulfide isomerase) were up-regulated while eight proteins (heat-shock gp96 precursor, nucleophosmin, ornithine aminotransferase, Ku70, l-arginine-glycine amidinotransferase, Smad anchor for receptor activation, ADP-ribosylation factor, WD repeat-containing protein isoform 1) were down-regulated by H. pylori infection in AGS cells. These proteins are related to cell proliferation, cell adhesion, carcinogenesis, cell-defense mechanisms against oxidative stress, membrane trafficking, and energy metabolism. Conclusions: Oxidative stress, cell proliferation, cell adhesion, and membrane trafficking may be involved in the pathogenesis of gastric diseases including cancer associated with H. pylori in a Korean isolate.",
author = "Cho, {Soon Ok} and Lim, {Joo Weon} and Jun, {Jong Ho} and Kim, {Kyung Hwan} and Hyeyoung Kim",
year = "2010",
month = "6",
day = "1",
doi = "10.1007/s10620-009-0908-z",
language = "English",
volume = "55",
pages = "1550--1564",
journal = "American Journal of Digestive Diseases",
issn = "0002-9211",
publisher = "Springer New York",
number = "6",

}

Helicobacter pylori in a Korean isolate expressed proteins differentially in human gastric epithelial cells. / Cho, Soon Ok; Lim, Joo Weon; Jun, Jong Ho; Kim, Kyung Hwan; Kim, Hyeyoung.

In: Digestive diseases and sciences, Vol. 55, No. 6, 01.06.2010, p. 1550-1564.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Helicobacter pylori in a Korean isolate expressed proteins differentially in human gastric epithelial cells

AU - Cho, Soon Ok

AU - Lim, Joo Weon

AU - Jun, Jong Ho

AU - Kim, Kyung Hwan

AU - Kim, Hyeyoung

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Purpose: The proteins expressed in gastric epithelial cells infected with Helicobacter pylori (H. pylori) may determine the clinical outcome such as chronic gastritis, peptic ulcer, and gastric carcinoma. The present study aims to determine the differentially expressed proteins in human gastric epithelial AGS cells that were infected with H. pylori in a Korean isolate, a cagA+, vacA s1b m2 iceA1 H. pylori by proteomic analysis. The differentially expressed proteins, whose expression levels were more or less than twofold in H. pylori-infected cells, were analyzed. Results: Ten proteins (chromatin assembly factor-1, proliferating cell nuclear antigen, 14-3-3 protein τ, eukaryotic translation initiation factor 6, heat-shock protein 90β, dimethylarginine dimethylaminohydrolase-1, l-lactate dehydrogenase B chain, prohibitin, triosephosphate isomerase, protein disulfide isomerase) were up-regulated while eight proteins (heat-shock gp96 precursor, nucleophosmin, ornithine aminotransferase, Ku70, l-arginine-glycine amidinotransferase, Smad anchor for receptor activation, ADP-ribosylation factor, WD repeat-containing protein isoform 1) were down-regulated by H. pylori infection in AGS cells. These proteins are related to cell proliferation, cell adhesion, carcinogenesis, cell-defense mechanisms against oxidative stress, membrane trafficking, and energy metabolism. Conclusions: Oxidative stress, cell proliferation, cell adhesion, and membrane trafficking may be involved in the pathogenesis of gastric diseases including cancer associated with H. pylori in a Korean isolate.

AB - Purpose: The proteins expressed in gastric epithelial cells infected with Helicobacter pylori (H. pylori) may determine the clinical outcome such as chronic gastritis, peptic ulcer, and gastric carcinoma. The present study aims to determine the differentially expressed proteins in human gastric epithelial AGS cells that were infected with H. pylori in a Korean isolate, a cagA+, vacA s1b m2 iceA1 H. pylori by proteomic analysis. The differentially expressed proteins, whose expression levels were more or less than twofold in H. pylori-infected cells, were analyzed. Results: Ten proteins (chromatin assembly factor-1, proliferating cell nuclear antigen, 14-3-3 protein τ, eukaryotic translation initiation factor 6, heat-shock protein 90β, dimethylarginine dimethylaminohydrolase-1, l-lactate dehydrogenase B chain, prohibitin, triosephosphate isomerase, protein disulfide isomerase) were up-regulated while eight proteins (heat-shock gp96 precursor, nucleophosmin, ornithine aminotransferase, Ku70, l-arginine-glycine amidinotransferase, Smad anchor for receptor activation, ADP-ribosylation factor, WD repeat-containing protein isoform 1) were down-regulated by H. pylori infection in AGS cells. These proteins are related to cell proliferation, cell adhesion, carcinogenesis, cell-defense mechanisms against oxidative stress, membrane trafficking, and energy metabolism. Conclusions: Oxidative stress, cell proliferation, cell adhesion, and membrane trafficking may be involved in the pathogenesis of gastric diseases including cancer associated with H. pylori in a Korean isolate.

UR - http://www.scopus.com/inward/record.url?scp=77954425332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954425332&partnerID=8YFLogxK

U2 - 10.1007/s10620-009-0908-z

DO - 10.1007/s10620-009-0908-z

M3 - Article

C2 - 19672714

AN - SCOPUS:77954425332

VL - 55

SP - 1550

EP - 1564

JO - American Journal of Digestive Diseases

JF - American Journal of Digestive Diseases

SN - 0002-9211

IS - 6

ER -