Helicobacter pylori outer membrane protein, HomC, shows geographic dependent polymorphism that is influenced by the Bab family

Aeryun Kim, Stephanie L. Servetas, Jieun Kang, Jinmoon Kim, Sungil Jang, Yun Hui Choi, Hanfu Su, Yeong Eui Jeon, Youngmin A. Hong, Yun Jung Yoo, D. Scott Merrell, Jeong Heon Cha

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H. pylori clinical isolates (AH) and 80 South Korean H. pylori clinical isolates (KH). In the current study, we expanded our investigation to include the less well characterized Hom family member, HomC. Overall, we identified and genotyped three homC variants: homCS, homCL, and homCM, in both populations. Similar to other polymorphic genes, the KH group showed less overall diversity, with 97.5% of strains harboring homCL. In contrast, a more heterogeneous profile was observed in strains derived from an American population; we found nearly equal distribution of homCS and homCL. Further analysis of the AH group identified associations between homC polymorphism and bab genotype; in AH strains, there was a significant association between homCL and carriage of babA at locus A. Since babA is an important virulence factor for the development of severe gastric disease, these data may suggest that homC polymorphism plays a role in H. pylori pathogenesis.

Original languageEnglish
Pages (from-to)846-852
Number of pages7
JournalJournal of Microbiology
Volume54
Issue number12
DOIs
Publication statusPublished - 2016 Dec 1

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Helicobacter pylori
Membrane Proteins
Immune Evasion
Stomach Diseases
Virulence Factors
Population
Disease Progression
Stomach
Genotype
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Kim, Aeryun ; Servetas, Stephanie L. ; Kang, Jieun ; Kim, Jinmoon ; Jang, Sungil ; Choi, Yun Hui ; Su, Hanfu ; Jeon, Yeong Eui ; Hong, Youngmin A. ; Yoo, Yun Jung ; Merrell, D. Scott ; Cha, Jeong Heon. / Helicobacter pylori outer membrane protein, HomC, shows geographic dependent polymorphism that is influenced by the Bab family. In: Journal of Microbiology. 2016 ; Vol. 54, No. 12. pp. 846-852.
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abstract = "The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H. pylori clinical isolates (AH) and 80 South Korean H. pylori clinical isolates (KH). In the current study, we expanded our investigation to include the less well characterized Hom family member, HomC. Overall, we identified and genotyped three homC variants: homCS, homCL, and homCM, in both populations. Similar to other polymorphic genes, the KH group showed less overall diversity, with 97.5{\%} of strains harboring homCL. In contrast, a more heterogeneous profile was observed in strains derived from an American population; we found nearly equal distribution of homCS and homCL. Further analysis of the AH group identified associations between homC polymorphism and bab genotype; in AH strains, there was a significant association between homCL and carriage of babA at locus A. Since babA is an important virulence factor for the development of severe gastric disease, these data may suggest that homC polymorphism plays a role in H. pylori pathogenesis.",
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Kim, A, Servetas, SL, Kang, J, Kim, J, Jang, S, Choi, YH, Su, H, Jeon, YE, Hong, YA, Yoo, YJ, Merrell, DS & Cha, JH 2016, 'Helicobacter pylori outer membrane protein, HomC, shows geographic dependent polymorphism that is influenced by the Bab family', Journal of Microbiology, vol. 54, no. 12, pp. 846-852. https://doi.org/10.1007/s12275-016-6434-8

Helicobacter pylori outer membrane protein, HomC, shows geographic dependent polymorphism that is influenced by the Bab family. / Kim, Aeryun; Servetas, Stephanie L.; Kang, Jieun; Kim, Jinmoon; Jang, Sungil; Choi, Yun Hui; Su, Hanfu; Jeon, Yeong Eui; Hong, Youngmin A.; Yoo, Yun Jung; Merrell, D. Scott; Cha, Jeong Heon.

In: Journal of Microbiology, Vol. 54, No. 12, 01.12.2016, p. 846-852.

Research output: Contribution to journalArticle

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T1 - Helicobacter pylori outer membrane protein, HomC, shows geographic dependent polymorphism that is influenced by the Bab family

AU - Kim, Aeryun

AU - Servetas, Stephanie L.

AU - Kang, Jieun

AU - Kim, Jinmoon

AU - Jang, Sungil

AU - Choi, Yun Hui

AU - Su, Hanfu

AU - Jeon, Yeong Eui

AU - Hong, Youngmin A.

AU - Yoo, Yun Jung

AU - Merrell, D. Scott

AU - Cha, Jeong Heon

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Y1 - 2016/12/1

N2 - The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H. pylori clinical isolates (AH) and 80 South Korean H. pylori clinical isolates (KH). In the current study, we expanded our investigation to include the less well characterized Hom family member, HomC. Overall, we identified and genotyped three homC variants: homCS, homCL, and homCM, in both populations. Similar to other polymorphic genes, the KH group showed less overall diversity, with 97.5% of strains harboring homCL. In contrast, a more heterogeneous profile was observed in strains derived from an American population; we found nearly equal distribution of homCS and homCL. Further analysis of the AH group identified associations between homC polymorphism and bab genotype; in AH strains, there was a significant association between homCL and carriage of babA at locus A. Since babA is an important virulence factor for the development of severe gastric disease, these data may suggest that homC polymorphism plays a role in H. pylori pathogenesis.

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