Hemodynamic Characteristics of Extracellular UTP in the Perfused Rat Liver

Indeok Kong, Hae Sook Chung, Kyusang Park, Joon Kyu Han, Joong Woo Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Uridine 5'-triphosphate (UTP) is stored in the granules of cells such as platelets and is released into the extracellular space upon cell stimulation. Extracellular UTP is known to influence many biological processes. We investigated the hemodynamic effects of UTP on the perfused rat liver and characterized its receptors. Liver perfusions were performed in a recirculation system under constant pressure (28 cmH2O). The perfusion flow and oxygen consumption rate were measured at 30 second intervals. UTP decreased the perfusion flow and the oxygen consumption rate, dose-dependently. UTP-induced changes were transient and disappeared in about 10 minutes. Suramin (P2-purinergic antagonist, 100 uM) and indomethacin (cyclooxygenase inhibitor, 20 uM) blocked UTP-induced hemodynamic changes significantly. The effects of UTP were also inhibited when Kupffer cells were damaged with treatment of gadolinium chloride (10 mg/kg iv). L-NAME (1 mM), a potent inhibitor of nitric oxide synthase, markedly enhanced and prolonged the contractile response of UTP in the hepatic vessel. These results suggest that UTP acts mainly on suramin-sensitive UTP receptors on the Kupffer cell through prostanoid synthesis. The nitric oxide systems in the endothelium seem to counteract the vasoconstrictile action of UTP in the hepatic circulation.

Original languageEnglish
Pages (from-to)262-269
Number of pages8
JournalYonsei medical journal
Volume37
Issue number4
DOIs
Publication statusPublished - 1996 Jan 1

Fingerprint

Uridine Triphosphate
Hemodynamics
Liver
Suramin
Kupffer Cells
Perfusion
Oxygen Consumption
Purinergic Antagonists
Liver Circulation
Biological Phenomena
Cyclooxygenase Inhibitors
NG-Nitroarginine Methyl Ester
Extracellular Space
Nitric Oxide Synthase
Indomethacin
Prostaglandins
Endothelium
Nitric Oxide
Blood Platelets
Pressure

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Kong, Indeok ; Chung, Hae Sook ; Park, Kyusang ; Han, Joon Kyu ; Lee, Joong Woo. / Hemodynamic Characteristics of Extracellular UTP in the Perfused Rat Liver. In: Yonsei medical journal. 1996 ; Vol. 37, No. 4. pp. 262-269.
@article{be59e55051e442a7a298b135cc85c033,
title = "Hemodynamic Characteristics of Extracellular UTP in the Perfused Rat Liver",
abstract = "Uridine 5'-triphosphate (UTP) is stored in the granules of cells such as platelets and is released into the extracellular space upon cell stimulation. Extracellular UTP is known to influence many biological processes. We investigated the hemodynamic effects of UTP on the perfused rat liver and characterized its receptors. Liver perfusions were performed in a recirculation system under constant pressure (28 cmH2O). The perfusion flow and oxygen consumption rate were measured at 30 second intervals. UTP decreased the perfusion flow and the oxygen consumption rate, dose-dependently. UTP-induced changes were transient and disappeared in about 10 minutes. Suramin (P2-purinergic antagonist, 100 uM) and indomethacin (cyclooxygenase inhibitor, 20 uM) blocked UTP-induced hemodynamic changes significantly. The effects of UTP were also inhibited when Kupffer cells were damaged with treatment of gadolinium chloride (10 mg/kg iv). L-NAME (1 mM), a potent inhibitor of nitric oxide synthase, markedly enhanced and prolonged the contractile response of UTP in the hepatic vessel. These results suggest that UTP acts mainly on suramin-sensitive UTP receptors on the Kupffer cell through prostanoid synthesis. The nitric oxide systems in the endothelium seem to counteract the vasoconstrictile action of UTP in the hepatic circulation.",
author = "Indeok Kong and Chung, {Hae Sook} and Kyusang Park and Han, {Joon Kyu} and Lee, {Joong Woo}",
year = "1996",
month = "1",
day = "1",
doi = "10.3349/ymj.1996.37.4.262",
language = "English",
volume = "37",
pages = "262--269",
journal = "Yonsei Medical Journal",
issn = "0513-5796",
publisher = "Yonsei University College of Medicine",
number = "4",

}

Hemodynamic Characteristics of Extracellular UTP in the Perfused Rat Liver. / Kong, Indeok; Chung, Hae Sook; Park, Kyusang; Han, Joon Kyu; Lee, Joong Woo.

In: Yonsei medical journal, Vol. 37, No. 4, 01.01.1996, p. 262-269.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hemodynamic Characteristics of Extracellular UTP in the Perfused Rat Liver

AU - Kong, Indeok

AU - Chung, Hae Sook

AU - Park, Kyusang

AU - Han, Joon Kyu

AU - Lee, Joong Woo

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Uridine 5'-triphosphate (UTP) is stored in the granules of cells such as platelets and is released into the extracellular space upon cell stimulation. Extracellular UTP is known to influence many biological processes. We investigated the hemodynamic effects of UTP on the perfused rat liver and characterized its receptors. Liver perfusions were performed in a recirculation system under constant pressure (28 cmH2O). The perfusion flow and oxygen consumption rate were measured at 30 second intervals. UTP decreased the perfusion flow and the oxygen consumption rate, dose-dependently. UTP-induced changes were transient and disappeared in about 10 minutes. Suramin (P2-purinergic antagonist, 100 uM) and indomethacin (cyclooxygenase inhibitor, 20 uM) blocked UTP-induced hemodynamic changes significantly. The effects of UTP were also inhibited when Kupffer cells were damaged with treatment of gadolinium chloride (10 mg/kg iv). L-NAME (1 mM), a potent inhibitor of nitric oxide synthase, markedly enhanced and prolonged the contractile response of UTP in the hepatic vessel. These results suggest that UTP acts mainly on suramin-sensitive UTP receptors on the Kupffer cell through prostanoid synthesis. The nitric oxide systems in the endothelium seem to counteract the vasoconstrictile action of UTP in the hepatic circulation.

AB - Uridine 5'-triphosphate (UTP) is stored in the granules of cells such as platelets and is released into the extracellular space upon cell stimulation. Extracellular UTP is known to influence many biological processes. We investigated the hemodynamic effects of UTP on the perfused rat liver and characterized its receptors. Liver perfusions were performed in a recirculation system under constant pressure (28 cmH2O). The perfusion flow and oxygen consumption rate were measured at 30 second intervals. UTP decreased the perfusion flow and the oxygen consumption rate, dose-dependently. UTP-induced changes were transient and disappeared in about 10 minutes. Suramin (P2-purinergic antagonist, 100 uM) and indomethacin (cyclooxygenase inhibitor, 20 uM) blocked UTP-induced hemodynamic changes significantly. The effects of UTP were also inhibited when Kupffer cells were damaged with treatment of gadolinium chloride (10 mg/kg iv). L-NAME (1 mM), a potent inhibitor of nitric oxide synthase, markedly enhanced and prolonged the contractile response of UTP in the hepatic vessel. These results suggest that UTP acts mainly on suramin-sensitive UTP receptors on the Kupffer cell through prostanoid synthesis. The nitric oxide systems in the endothelium seem to counteract the vasoconstrictile action of UTP in the hepatic circulation.

UR - http://www.scopus.com/inward/record.url?scp=0030202933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030202933&partnerID=8YFLogxK

U2 - 10.3349/ymj.1996.37.4.262

DO - 10.3349/ymj.1996.37.4.262

M3 - Article

C2 - 8942296

AN - SCOPUS:0030202933

VL - 37

SP - 262

EP - 269

JO - Yonsei Medical Journal

JF - Yonsei Medical Journal

SN - 0513-5796

IS - 4

ER -