Hemoglobin assay in anemic patients with a photothermal spectral-domain optical coherence reflectometric sensor

Hun Kim, Sungwook Song, Jinyeong Yim, Hyun Ok Kim, Chulmin Joo

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Background: We recently introduced an optical sensor, termed photothermal spectral-domain optical coherence reflectometer (PT SD-OCR), capable of direct measurement of hemoglobin concentration ([Hb]) without chemicals and pre-processing of blood. PT SD-OCR measures [Hb] by quantifying refractive index changes of blood samples due to photothermal effect under the irradiation of 532-nm light. We evaluated diagnostic accuracy and precision of PT SD-OCR in anemic patients. Methods: We used PT SD-OCR to measure [Hb] in 50 anemic patients, and examined its accuracy against a hematology analyzer (ADVIA® 2120i, Siemens AG). Its precision was assessed by examining the SD and CV based on 20 repeated measurements on 3 blood samples. Its performance was also compared with a hemoglobinometer (HemoCue® 201. +, HemoCue®). Results: The PT SD-OCR demonstrated good correspondence with the hematology analyzer with a Pearson's correlation coefficient of 0.992 and a bias of - 0.055. g/dl. Standard deviation and CV were measured to be <. 0.25. g/dl and <. 2.05%. Conclusions: Simple and chemical-free operation of PT SD-OCR enabled rapid and accurate measurement of [Hb] in anemic patients. The sensor is expected to facilitate clinical procedures related to blood-related disorders in patient care.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalClinica Chimica Acta
Volume439
DOIs
Publication statusPublished - 2014 Oct 12

Bibliographical note

Funding Information:
The authors thank Soocheol Kim and Suho Ryu for their help with data analysis and critical reading of the manuscript. This research was supported in part by the research programs of National Research Foundation of Korea (NRF) (MEST No. NRF-2012R1A1A1003867 and NRF-2010-0020790 ).

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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