Hemoglobin assay in anemic patients with a photothermal spectral-domain optical coherence reflectometric sensor

Hun Kim, Sungwook Song, Jinyeong Yim, Hyun Ok Kim, Chulmin Joo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: We recently introduced an optical sensor, termed photothermal spectral-domain optical coherence reflectometer (PT SD-OCR), capable of direct measurement of hemoglobin concentration ([Hb]) without chemicals and pre-processing of blood. PT SD-OCR measures [Hb] by quantifying refractive index changes of blood samples due to photothermal effect under the irradiation of 532-nm light. We evaluated diagnostic accuracy and precision of PT SD-OCR in anemic patients. Methods: We used PT SD-OCR to measure [Hb] in 50 anemic patients, and examined its accuracy against a hematology analyzer (ADVIA® 2120i, Siemens AG). Its precision was assessed by examining the SD and CV based on 20 repeated measurements on 3 blood samples. Its performance was also compared with a hemoglobinometer (HemoCue® 201. +, HemoCue®). Results: The PT SD-OCR demonstrated good correspondence with the hematology analyzer with a Pearson's correlation coefficient of 0.992 and a bias of - 0.055. g/dl. Standard deviation and CV were measured to be <. 0.25. g/dl and <. 2.05%. Conclusions: Simple and chemical-free operation of PT SD-OCR enabled rapid and accurate measurement of [Hb] in anemic patients. The sensor is expected to facilitate clinical procedures related to blood-related disorders in patient care.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalClinica Chimica Acta
Volume439
DOIs
Publication statusPublished - 2014 Oct 12

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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