Hepatitis C virus core protein inhibits interleukin 12 and nitric oxide production from activated macrophages

Chu Hee Lee, Yo Han Choi, Se Hwan Yang, Chang Woo Lee, Sang-Jun Ha, Young Chul Sung

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

A characteristic feature of hepatitis C virus (HCV) infection is a high frequency of persistence and the progression to chronic liver diseases. Recent data suggest that prevalent T helper (Th) 2 immunity as well as weak HCV-specific T-cell response is associated with viral persistence. Here, we showed that the production of interleukin 12 (IL-12) and nitric oxide (NO) that is critical for the induction of Th1 and innate immunity, but not that of tumor necrosis factor α (TNF-α), was significantly suppressed in both HCV core-expressing macrophage cell lines and mouse peritoneal macrophages treated with recombinant core protein. In addition, IL-12 p40 promoter activity was repressed by the presence of HCV core in macrophages stimulated with lipopolysaccharride (LPS) following IFN-γ treatment, indicating that IL-12 production may be downregulated at the transcriptional level. We also found that proliferation of T cells and IFN-γ, production in mixed lymphocyte reactions (MLR) with core-expressing cells were inhibited. Taken together, our results suggest that HCV core protein could play roles in suppressing the induction of Th1 immunity through inhibition of IL-12 and NO production.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalVirology
Volume279
Issue number1
DOIs
Publication statusPublished - 2001 Jan 20

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Interleukin-12
Hepacivirus
Nitric Oxide
Macrophages
Immunity
T-Lymphocytes
Mixed Lymphocyte Culture Test
Peritoneal Macrophages
Virus Diseases
Recombinant Proteins
Innate Immunity
Liver Diseases
Chronic Disease
Down-Regulation
Tumor Necrosis Factor-alpha
Cell Line
Hepatitis C virus nucleocapsid protein

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Lee, Chu Hee ; Choi, Yo Han ; Yang, Se Hwan ; Lee, Chang Woo ; Ha, Sang-Jun ; Sung, Young Chul. / Hepatitis C virus core protein inhibits interleukin 12 and nitric oxide production from activated macrophages. In: Virology. 2001 ; Vol. 279, No. 1. pp. 271-279.
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Hepatitis C virus core protein inhibits interleukin 12 and nitric oxide production from activated macrophages. / Lee, Chu Hee; Choi, Yo Han; Yang, Se Hwan; Lee, Chang Woo; Ha, Sang-Jun; Sung, Young Chul.

In: Virology, Vol. 279, No. 1, 20.01.2001, p. 271-279.

Research output: Contribution to journalArticle

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