Hepatoprotective dibenzylbutyrolactone lignans of Torreya nucifera against CCI4-induced toxicity in primary cultured rat hepatocytes

Seung Hyun Kim, Young Pyo Jang, Sang Hyun Sung, Chang Jong Kim, Jin Woong Kim, Young Choong Kim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Three dibenzylbutyrolactone lignans, (-)-arctigenin, (-)-traxillagenin, and (-)-4′-demethyltraxillagenin, isolated from the bark of Torreya nucifera SIEB. et ZUCC. (Taxaceae) showed significant hepatoprotective activity in primary cultures of rat hepatocytes injured by carbon tetrachloride (CCl 4). These lignans reduced the release of glutamic pyruvic transaminase into the culture medium from the CCl4-injured primary cultures of rat hepatocytes. Further investigation revealed that the three lignans significantly preserved the level of glutathione (GSH) and activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase in the CCl4-injured rat hepatocytes. The lignans also ameliorated lipid peroxidation as demonstrated by a reduction in malondialdehyde-related products. Moreover, these lignans significantly attenuated the GSH reduction caused by diethylmaleate which depletes GSH through the formation of stable conjugates. However, these lignans showed no effect on the GSH synthesis inhibited by buthionine sulfoximine. From these results, it can be concluded that arctigenin, traxillagenin, and 4′- demethyltraxillagenin may protect hepatocytes from CCl4 injury by maintaining the GSH level.

Original languageEnglish
Pages (from-to)1202-1205
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume26
Issue number8
DOIs
Publication statusPublished - 2003 Jan 1

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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