Abstract
Background: Our previous study suggested that licorice has anti-inflammatory activity in lipopolysaccharide-stimulated microglial cells and anti-oxidative activity in tert-butyl hydroperoxide-induced oxidative liver damage. In this study, we evaluated the effect of licorice on chronic alcohol-induced fatty liver injury mediated by inflammation and oxidative stress. Methods: Raw licorice was extracted, and quantitative and qualitative analysis of its components was performed by using LC-MS/MS. Mice were fed a liquid alcohol diet with or without licorice for 4weeks. Results: We have standardized 70% fermented ethanol extracted licorice and confirmed by LC-MS/MS as glycyrrhizic acid (GA), 15.77±0.34μg/mg; liquiritin (LQ), 14.55±0.42μg/mg; and liquiritigenin (LG), 1.34±0.02μg/mg, respectively. Alcohol consumption increased serum alanine aminotransferase and aspartate aminotransferase activities and the levels of triglycerides and tumor necrosis factor (TNF)-aα. Lipid accumulation in the liver was also markedly induced, whereas the glutathione level was reduced. All these alcohol-induced changes were effectively inhibited by licorice treatment. In particular, the hepatic glutathione level was restored and alcohol-induced TNF-aα production was significantly inhibited by licorice. Conclusion: Taken together, our data suggests that protective effect of licorice against alcohol-induced liver injury may be attributed to its anti-inflammatory activity and enhancement of antioxidant defense.
| Original language | English |
|---|---|
| Article number | 19 |
| Journal | BMC Complementary and Alternative Medicine |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2016 Jan 22 |
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All Science Journal Classification (ASJC) codes
- Complementary and alternative medicine
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Hepatoprotective effect of licorice, the root of Glycyrrhiza uralensis Fischer, in alcohol-induced fatty liver disease. / Jung, Jae Chul; Lee, Yun-Hee; Kim, Sou Hyun; Kim, Keuk Jun; Kim, Kyung Mi; Oh, Seikwan; Jung, Young Suk.
In: BMC Complementary and Alternative Medicine, Vol. 16, No. 1, 19, 22.01.2016.Research output: Contribution to journal › Article
TY - JOUR
T1 - Hepatoprotective effect of licorice, the root of Glycyrrhiza uralensis Fischer, in alcohol-induced fatty liver disease
AU - Jung, Jae Chul
AU - Lee, Yun-Hee
AU - Kim, Sou Hyun
AU - Kim, Keuk Jun
AU - Kim, Kyung Mi
AU - Oh, Seikwan
AU - Jung, Young Suk
PY - 2016/1/22
Y1 - 2016/1/22
N2 - Background: Our previous study suggested that licorice has anti-inflammatory activity in lipopolysaccharide-stimulated microglial cells and anti-oxidative activity in tert-butyl hydroperoxide-induced oxidative liver damage. In this study, we evaluated the effect of licorice on chronic alcohol-induced fatty liver injury mediated by inflammation and oxidative stress. Methods: Raw licorice was extracted, and quantitative and qualitative analysis of its components was performed by using LC-MS/MS. Mice were fed a liquid alcohol diet with or without licorice for 4weeks. Results: We have standardized 70% fermented ethanol extracted licorice and confirmed by LC-MS/MS as glycyrrhizic acid (GA), 15.77±0.34μg/mg; liquiritin (LQ), 14.55±0.42μg/mg; and liquiritigenin (LG), 1.34±0.02μg/mg, respectively. Alcohol consumption increased serum alanine aminotransferase and aspartate aminotransferase activities and the levels of triglycerides and tumor necrosis factor (TNF)-aα. Lipid accumulation in the liver was also markedly induced, whereas the glutathione level was reduced. All these alcohol-induced changes were effectively inhibited by licorice treatment. In particular, the hepatic glutathione level was restored and alcohol-induced TNF-aα production was significantly inhibited by licorice. Conclusion: Taken together, our data suggests that protective effect of licorice against alcohol-induced liver injury may be attributed to its anti-inflammatory activity and enhancement of antioxidant defense.
AB - Background: Our previous study suggested that licorice has anti-inflammatory activity in lipopolysaccharide-stimulated microglial cells and anti-oxidative activity in tert-butyl hydroperoxide-induced oxidative liver damage. In this study, we evaluated the effect of licorice on chronic alcohol-induced fatty liver injury mediated by inflammation and oxidative stress. Methods: Raw licorice was extracted, and quantitative and qualitative analysis of its components was performed by using LC-MS/MS. Mice were fed a liquid alcohol diet with or without licorice for 4weeks. Results: We have standardized 70% fermented ethanol extracted licorice and confirmed by LC-MS/MS as glycyrrhizic acid (GA), 15.77±0.34μg/mg; liquiritin (LQ), 14.55±0.42μg/mg; and liquiritigenin (LG), 1.34±0.02μg/mg, respectively. Alcohol consumption increased serum alanine aminotransferase and aspartate aminotransferase activities and the levels of triglycerides and tumor necrosis factor (TNF)-aα. Lipid accumulation in the liver was also markedly induced, whereas the glutathione level was reduced. All these alcohol-induced changes were effectively inhibited by licorice treatment. In particular, the hepatic glutathione level was restored and alcohol-induced TNF-aα production was significantly inhibited by licorice. Conclusion: Taken together, our data suggests that protective effect of licorice against alcohol-induced liver injury may be attributed to its anti-inflammatory activity and enhancement of antioxidant defense.
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U2 - 10.1186/s12906-016-0997-0
DO - 10.1186/s12906-016-0997-0
M3 - Article
C2 - 26801973
AN - SCOPUS:84955353038
VL - 16
JO - BMC Complementary and Alternative Medicine
JF - BMC Complementary and Alternative Medicine
SN - 1472-6882
IS - 1
M1 - 19
ER -