Abstract
Summary of Background Data: Spinal stenosis is caused, in part, by hypertrophy and ossification of the LF, which are induced by aging and degenerative process. It is well known that degenerated IVDs spontaneously produce inflammatory cytokines. Therefore, we hypothesized that degenerated IVD may affect adjacent LF through secreted inflammatory cytokines.
Methods: LF and herniated lumbar IVD tissues were obtained during surgical spinal procedures. LF fibroblasts were isolated by enzymatic digestion of LF tissue. LF cell cultures were treated with disk supernatant from herniated IVDs. Secreted cytokines from IVD tissue culture were detected by enzymelinked immunosorbent assay. After analysis of cytotoxicity, DNA synthesis was measured. Reverse transcription-polymerase chain reaction for mRNA expressions of types I, II, III, V, and XI collagen and osteocalcin, and histochemical stains were performed.
Results: Supernatant from tissue culture of herniated IVD showed increased production of interleukin-1a, interleukin-6, tumor necrosis factor-α, prostaglandin E 2 , and nitric oxide compared with disk tissue culture from traumatic condition. There was no cytotoxicity in LF cells treated with disk supernatant from herniated IVDs. There was significant increase in DNA synthesis, upregulation in mRNA expression of types III, XI collagen and osteocalcin, whereas variable expression pattern of type I and V, and strong positive stains for Von Kossa and alkaline phosphatase in LF cultures with disk supernatant.
Conclusions: Degenerated and herniated IVDs provide an important pathomechanism in hypertrophy and ossification of the LF through inflammatory cytokines.
Study Design: In vitro experiment using degenerated human ligamentum flavum (LF) and herniated intervertebral disk (IVD).
Objectives: To investigate the role and effect of degenerated and herniated IVDs on LF hypertrophy and ossification.
| Original language | English |
|---|---|
| Pages (from-to) | 382-389 |
| Number of pages | 8 |
| Journal | Journal of Spinal Disorders and Techniques |
| Volume | 27 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Surgery
- Orthopedics and Sports Medicine
- Clinical Neurology
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Herniated intervertebral disk induces hypertrophy and ossification of ligamentum flavum. / Kang, Young Mi; Suk, Kyung Soo; Lee, Byung Ho; Kim, Hak Sun; Lee, Kwang Il; Park, Si Young; Lee, Hwan Mo; moon, seonghwan.
In: Journal of Spinal Disorders and Techniques, Vol. 27, No. 7, 01.01.2014, p. 382-389.Research output: Contribution to journal › Article
TY - JOUR
T1 - Herniated intervertebral disk induces hypertrophy and ossification of ligamentum flavum
AU - Kang, Young Mi
AU - Suk, Kyung Soo
AU - Lee, Byung Ho
AU - Kim, Hak Sun
AU - Lee, Kwang Il
AU - Park, Si Young
AU - Lee, Hwan Mo
AU - moon, seonghwan
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Summary of Background Data: Spinal stenosis is caused, in part, by hypertrophy and ossification of the LF, which are induced by aging and degenerative process. It is well known that degenerated IVDs spontaneously produce inflammatory cytokines. Therefore, we hypothesized that degenerated IVD may affect adjacent LF through secreted inflammatory cytokines.Methods: LF and herniated lumbar IVD tissues were obtained during surgical spinal procedures. LF fibroblasts were isolated by enzymatic digestion of LF tissue. LF cell cultures were treated with disk supernatant from herniated IVDs. Secreted cytokines from IVD tissue culture were detected by enzymelinked immunosorbent assay. After analysis of cytotoxicity, DNA synthesis was measured. Reverse transcription-polymerase chain reaction for mRNA expressions of types I, II, III, V, and XI collagen and osteocalcin, and histochemical stains were performed. Results: Supernatant from tissue culture of herniated IVD showed increased production of interleukin-1a, interleukin-6, tumor necrosis factor-α, prostaglandin E 2 , and nitric oxide compared with disk tissue culture from traumatic condition. There was no cytotoxicity in LF cells treated with disk supernatant from herniated IVDs. There was significant increase in DNA synthesis, upregulation in mRNA expression of types III, XI collagen and osteocalcin, whereas variable expression pattern of type I and V, and strong positive stains for Von Kossa and alkaline phosphatase in LF cultures with disk supernatant. Conclusions: Degenerated and herniated IVDs provide an important pathomechanism in hypertrophy and ossification of the LF through inflammatory cytokines.Study Design: In vitro experiment using degenerated human ligamentum flavum (LF) and herniated intervertebral disk (IVD).Objectives: To investigate the role and effect of degenerated and herniated IVDs on LF hypertrophy and ossification.
AB - Summary of Background Data: Spinal stenosis is caused, in part, by hypertrophy and ossification of the LF, which are induced by aging and degenerative process. It is well known that degenerated IVDs spontaneously produce inflammatory cytokines. Therefore, we hypothesized that degenerated IVD may affect adjacent LF through secreted inflammatory cytokines.Methods: LF and herniated lumbar IVD tissues were obtained during surgical spinal procedures. LF fibroblasts were isolated by enzymatic digestion of LF tissue. LF cell cultures were treated with disk supernatant from herniated IVDs. Secreted cytokines from IVD tissue culture were detected by enzymelinked immunosorbent assay. After analysis of cytotoxicity, DNA synthesis was measured. Reverse transcription-polymerase chain reaction for mRNA expressions of types I, II, III, V, and XI collagen and osteocalcin, and histochemical stains were performed. Results: Supernatant from tissue culture of herniated IVD showed increased production of interleukin-1a, interleukin-6, tumor necrosis factor-α, prostaglandin E 2 , and nitric oxide compared with disk tissue culture from traumatic condition. There was no cytotoxicity in LF cells treated with disk supernatant from herniated IVDs. There was significant increase in DNA synthesis, upregulation in mRNA expression of types III, XI collagen and osteocalcin, whereas variable expression pattern of type I and V, and strong positive stains for Von Kossa and alkaline phosphatase in LF cultures with disk supernatant. Conclusions: Degenerated and herniated IVDs provide an important pathomechanism in hypertrophy and ossification of the LF through inflammatory cytokines.Study Design: In vitro experiment using degenerated human ligamentum flavum (LF) and herniated intervertebral disk (IVD).Objectives: To investigate the role and effect of degenerated and herniated IVDs on LF hypertrophy and ossification.
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U2 - 10.1097/BSD.0b013e3182a26532
DO - 10.1097/BSD.0b013e3182a26532
M3 - Article
VL - 27
SP - 382
EP - 389
JO - Journal of Spinal Disorders
JF - Journal of Spinal Disorders
SN - 1536-0652
IS - 7
ER -