Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II

So Hee Kwon, Laurence Florens, Selene K. Swanson, Michael P. Washburn, Susan M. Abmayr, Jerry L. Workman

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Heterochromatin protein 1 (HP1) is well known as a silencing protein found at pericentric heterochromatin. Most eukaryotes have at least three isoforms of HP1 that play differential roles in heterochromatin and euchromatin. In addition to its role in heterochromatin, HP1 proteins have been shown to function in transcription elongation. To gain insights into the transcription functions of HP1, we sought to identify novel HP1-interacting proteins. Biochemical and proteomic approaches revealed that HP1 interacts with the histone chaperone complex FACT (facilitates chromatin transcription). HP1c interacts with the SSRP1 (structure-specific recognition protein 1) subunit and the intact FACT complex. Moreover, HP1c guides the recruitment of FACT to active genes and links FACT to active forms of RNA polymerase II. The absence of HP1c partially impairs the recruitment of FACT into heat-shock loci and causes a defect in heat-shock gene expression. Thus, HP1c functions to recruit the FACT complex to RNA polymerase II.

Original languageEnglish
Pages (from-to)2133-2145
Number of pages13
JournalGenes and Development
Volume24
Issue number19
DOIs
Publication statusPublished - 2010 Oct 1

Fingerprint

Histone Chaperones
RNA Polymerase II
Chromatin
Heterochromatin
Shock
Hot Temperature
Euchromatin
Proteins
Protein Subunits
Eukaryota
Proteomics
heterochromatin-specific nonhistone chromosomal protein HP-1
Protein Isoforms
Gene Expression

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Medicine(all)

Cite this

Kwon, So Hee ; Florens, Laurence ; Swanson, Selene K. ; Washburn, Michael P. ; Abmayr, Susan M. ; Workman, Jerry L. / Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II. In: Genes and Development. 2010 ; Vol. 24, No. 19. pp. 2133-2145.
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Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II. / Kwon, So Hee; Florens, Laurence; Swanson, Selene K.; Washburn, Michael P.; Abmayr, Susan M.; Workman, Jerry L.

In: Genes and Development, Vol. 24, No. 19, 01.10.2010, p. 2133-2145.

Research output: Contribution to journalArticle

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