Heterogeneous virulence potential and high antibiotic resistance of Pseudomonas aeruginosa strains isolated from Korean pneumonia patients

Mi Young Yoon, Kang Mu Lee, Seokhoon Jeong, Jungmin Kim, Sang Sun Yoon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is an opportunistic human pathogen of clinical importance that causes airway infections in immunocompromised patients. Here, we report the virulence-associated characteristics of strains of P. aeruginosa, isolated from the sputa of 25 Korean pneumonia patients. A high degree of genomic plasticity was observed by random amplified polymorphic DNA genotype analysis, suggesting that the infections were caused by strains with diverse genomic backgrounds. Biofilm formation of each isolate was heterogeneous in terms of their relative motilities. In addition, 48% of isolates were defective in the production of 3-oxo-C12-HSL (PAI-1), a quorum sensing signal molecule. In these strains, PAI-1-dependent elastase production was correspondingly decreased, suggesting that a large number of strains were presumed to be quorum sensing deficient. Multidrug resistance (MDR) was seen in 56% of the isolates tested, and 44% of the MDR strains were resistant to five or more antibiotics. Taken together, our results provide additional insights into the virulence traits of P. aeruginosa clinical isolates, which will aid in treating P. aeruginosa infections in pneumonia patients.

Original languageEnglish
Pages (from-to)518-525
Number of pages8
JournalJournal of Microbiology
Volume48
Issue number4
DOIs
Publication statusPublished - 2010 Sep 3

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Microbial Drug Resistance
Pseudomonas aeruginosa
Virulence
Pneumonia
Quorum Sensing
Plasminogen Activator Inhibitor 1
Multiple Drug Resistance
Pseudomonas Infections
Pancreatic Elastase
Immunocompromised Host
Biofilms
Infection
Sputum
Genotype
Anti-Bacterial Agents
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "Pseudomonas aeruginosa is an opportunistic human pathogen of clinical importance that causes airway infections in immunocompromised patients. Here, we report the virulence-associated characteristics of strains of P. aeruginosa, isolated from the sputa of 25 Korean pneumonia patients. A high degree of genomic plasticity was observed by random amplified polymorphic DNA genotype analysis, suggesting that the infections were caused by strains with diverse genomic backgrounds. Biofilm formation of each isolate was heterogeneous in terms of their relative motilities. In addition, 48{\%} of isolates were defective in the production of 3-oxo-C12-HSL (PAI-1), a quorum sensing signal molecule. In these strains, PAI-1-dependent elastase production was correspondingly decreased, suggesting that a large number of strains were presumed to be quorum sensing deficient. Multidrug resistance (MDR) was seen in 56{\%} of the isolates tested, and 44{\%} of the MDR strains were resistant to five or more antibiotics. Taken together, our results provide additional insights into the virulence traits of P. aeruginosa clinical isolates, which will aid in treating P. aeruginosa infections in pneumonia patients.",
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Heterogeneous virulence potential and high antibiotic resistance of Pseudomonas aeruginosa strains isolated from Korean pneumonia patients. / Yoon, Mi Young; Lee, Kang Mu; Jeong, Seokhoon; Kim, Jungmin; Yoon, Sang Sun.

In: Journal of Microbiology, Vol. 48, No. 4, 03.09.2010, p. 518-525.

Research output: Contribution to journalArticle

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