Hexokinase 2 is a molecular bridge linking telomerase and autophagy

Jae Il Roh, Yujin Kim, Jahyun Oh, Yunmi Kim, Jeehyun Lee, Jaehoon Lee, Kyung Hee Chun, Han Woong Lee

Research output: Contribution to journalArticle

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Abstract

Autophagy is systematically regulated by upstream factors and nutrients. Recent studies reported that telomerase and hexokinase 2 [HK2) regulate autophagy through mTOR and that telomerase has the capacity to bind to the HK2 promoter. However, the molecular linkage among telomerase, HK2, and autophagy is not fully understood. Here, we show that HK2 connects telomerase to autophagy. HK2 inhibition in HepG2 cells suppressed TERT-induced autophagy activation and further enhancement by glucose deprivation. The HK2 downstream factor mTOR was responsible for the TERT-induced autophagy activation under glucose deprivation, implying that TERT promotes autophagy through an HK2-mTOR pathway. TERC played a role similar to that of TERT, and simultaneous expression of TERT and TERC synergistically enhanced HK2 expression and autophagy. At the gene level, TERT bound to the HK2 promoter at a specific region harboring the telomerase-responsive sequence ‘TTGGG.’ Mutagenesis of TERC and the TERT-responsive element in the HK2 promoter revealed that TERC is required for the binding of TERT to the HK2 promoter. We demonstrate the existence of a telomerase-HK2-mTOR-autophagy axis and suggest that inhibition of the interaction between telomerase and the HK2 promoter diminishes glucose starvation-induced autophagy.

Original languageEnglish
Article numbere0193182
JournalPloS one
Volume13
Issue number2
DOIs
Publication statusPublished - 2018 Feb

Fingerprint

telomerase
Hexokinase
hexokinase
autophagy
Telomerase
Autophagy
promoter regions
Glucose
glucose
Chemical activation
Mutagenesis
Hep G2 Cells
Starvation
mutagenesis
Nutrients
linkage (genetics)
starvation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Roh, Jae Il ; Kim, Yujin ; Oh, Jahyun ; Kim, Yunmi ; Lee, Jeehyun ; Lee, Jaehoon ; Chun, Kyung Hee ; Lee, Han Woong. / Hexokinase 2 is a molecular bridge linking telomerase and autophagy. In: PloS one. 2018 ; Vol. 13, No. 2.
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abstract = "Autophagy is systematically regulated by upstream factors and nutrients. Recent studies reported that telomerase and hexokinase 2 [HK2) regulate autophagy through mTOR and that telomerase has the capacity to bind to the HK2 promoter. However, the molecular linkage among telomerase, HK2, and autophagy is not fully understood. Here, we show that HK2 connects telomerase to autophagy. HK2 inhibition in HepG2 cells suppressed TERT-induced autophagy activation and further enhancement by glucose deprivation. The HK2 downstream factor mTOR was responsible for the TERT-induced autophagy activation under glucose deprivation, implying that TERT promotes autophagy through an HK2-mTOR pathway. TERC played a role similar to that of TERT, and simultaneous expression of TERT and TERC synergistically enhanced HK2 expression and autophagy. At the gene level, TERT bound to the HK2 promoter at a specific region harboring the telomerase-responsive sequence ‘TTGGG.’ Mutagenesis of TERC and the TERT-responsive element in the HK2 promoter revealed that TERC is required for the binding of TERT to the HK2 promoter. We demonstrate the existence of a telomerase-HK2-mTOR-autophagy axis and suggest that inhibition of the interaction between telomerase and the HK2 promoter diminishes glucose starvation-induced autophagy.",
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Roh, JI, Kim, Y, Oh, J, Kim, Y, Lee, J, Lee, J, Chun, KH & Lee, HW 2018, 'Hexokinase 2 is a molecular bridge linking telomerase and autophagy', PloS one, vol. 13, no. 2, e0193182. https://doi.org/10.1371/journal.pone.0193182

Hexokinase 2 is a molecular bridge linking telomerase and autophagy. / Roh, Jae Il; Kim, Yujin; Oh, Jahyun; Kim, Yunmi; Lee, Jeehyun; Lee, Jaehoon; Chun, Kyung Hee; Lee, Han Woong.

In: PloS one, Vol. 13, No. 2, e0193182, 02.2018.

Research output: Contribution to journalArticle

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