Capturing virus aerosols in a small volume of liquid is essential when monitoring airborne viruses. As such, aerosol-to-hydrosol enrichment is required to produce a detectable viral sample for real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. To meet this requirement, the efficient and non-destructive collection of airborne virus particles is needed, while the incoming air flow rate should be sufficiently high to quickly collect a large number of virus particles. To achieve this, we introduced a high air flow-rate electrostatic sampler (HAFES) that collected virus aerosols (human coronavirus 229E, influenza A virus subtypes H1N1 and H3N2, and bacteriophage MS2) in a continuously flowing liquid. Viral collection efficiency was evaluated using aerosol particle counts, while viral recovery rates were assessed using real-time qRT-PCR and plaque assays. An air sampling period of 20 min was sufficient to produce a sample suitable for use in real-time qRT-PCR in a viral epidemic scenario.
Bibliographical noteFunding Information:
This work was supported by the Technology Innovation Program-Industrial Technology Alchemist Project ( 20012215 , Intelligent platform for in-situ virus detection and analysis) funded by the Ministry of Trade, Industry and Energy, Korea (MOTIE, Korea).
© 2021 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Environmental Chemistry
- Waste Management and Disposal
- Health, Toxicology and Mutagenesis