High-density array of single cell traps for high-throughput imaging of calcium dynamics in response to oxidative stress

Catherine A. Rivet, Kwanghun Chung, Melissa L. Kemp, Hang Lu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Stochasticity in gene expression, protein or metabolite levels contributes to cell-cell variations. The analysis of these variations could lead to a better understanding of cellular processes and drug responses, but require single cell analysis techniques. To this end, we developed a new microfluidic platform for high-throughput capture (in about a minute) with low shear and imaging of thousands of single cells. This platform is compatible with upstream components for complex cell stimulation patterns. Imaging of calcium dynamics in response to oxidative stress with this platform reveals variable phenotypic responses within clonal populations.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages2041-2043
Number of pages3
Publication statusPublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume3

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Rivet, C. A., Chung, K., Kemp, M. L., & Lu, H. (2010). High-density array of single cell traps for high-throughput imaging of calcium dynamics in response to oxidative stress. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 2041-2043). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 3).