TY - GEN
T1 - High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis
AU - Cheng, Yu Heng
AU - Chen, Yu Chih
AU - Ingram, Patrick
AU - Yoon, Euisik
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - We present a high-efficiency single-cell capture scheme utilizing herringbone vortices for small assay samples consisting of only tens of cells. In our previous work of hydrodynamic cell capture, the laminar flow with abrupt expansion in a mircowell restricts the capture efficiency by diverting cells away from the capture site, which results in low capture rate. In this work, we used herringbone vortices to steer cells towards the capture site to facilitate cell capture efficiency. The proposed design achieves ∼65% capture efficiency when loading only 20, 40 and 60 MDA-MB-321 (breast cancer) cells. Compared to the capture efficiency of ∼35% without the herringbone, the proposed cell-focusing capture scheme demonstrates the feasibility for small samples analysis.
AB - We present a high-efficiency single-cell capture scheme utilizing herringbone vortices for small assay samples consisting of only tens of cells. In our previous work of hydrodynamic cell capture, the laminar flow with abrupt expansion in a mircowell restricts the capture efficiency by diverting cells away from the capture site, which results in low capture rate. In this work, we used herringbone vortices to steer cells towards the capture site to facilitate cell capture efficiency. The proposed design achieves ∼65% capture efficiency when loading only 20, 40 and 60 MDA-MB-321 (breast cancer) cells. Compared to the capture efficiency of ∼35% without the herringbone, the proposed cell-focusing capture scheme demonstrates the feasibility for small samples analysis.
UR - http://www.scopus.com/inward/record.url?scp=84907364040&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907364040&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907364040
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 350
EP - 352
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -