Abstract
We present a high-efficiency single-cell capture scheme utilizing herringbone vortices for small assay samples consisting of only tens of cells. In our previous work of hydrodynamic cell capture, the laminar flow with abrupt expansion in a mircowell restricts the capture efficiency by diverting cells away from the capture site, which results in low capture rate. In this work, we used herringbone vortices to steer cells towards the capture site to facilitate cell capture efficiency. The proposed design achieves ∼65% capture efficiency when loading only 20, 40 and 60 MDA-MB-321 (breast cancer) cells. Compared to the capture efficiency of ∼35% without the herringbone, the proposed cell-focusing capture scheme demonstrates the feasibility for small samples analysis.
| Original language | English |
|---|---|
| Title of host publication | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 350-352 |
| Number of pages | 3 |
| ISBN (Print) | 9781632666246 |
| Publication status | Published - 2013 Jan 1 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: 2013 Oct 27 → 2013 Oct 31 |
Publication series
| Name | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Volume | 1 |
Other
| Other | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Country | Germany |
| City | Freiburg |
| Period | 13/10/27 → 13/10/31 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
Cite this
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High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis. / Cheng, Yu Heng; Chen, Yu Chih; Ingram, Patrick; Yoon, Euisik.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 350-352 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis
AU - Cheng, Yu Heng
AU - Chen, Yu Chih
AU - Ingram, Patrick
AU - Yoon, Euisik
PY - 2013/1/1
Y1 - 2013/1/1
N2 - We present a high-efficiency single-cell capture scheme utilizing herringbone vortices for small assay samples consisting of only tens of cells. In our previous work of hydrodynamic cell capture, the laminar flow with abrupt expansion in a mircowell restricts the capture efficiency by diverting cells away from the capture site, which results in low capture rate. In this work, we used herringbone vortices to steer cells towards the capture site to facilitate cell capture efficiency. The proposed design achieves ∼65% capture efficiency when loading only 20, 40 and 60 MDA-MB-321 (breast cancer) cells. Compared to the capture efficiency of ∼35% without the herringbone, the proposed cell-focusing capture scheme demonstrates the feasibility for small samples analysis.
AB - We present a high-efficiency single-cell capture scheme utilizing herringbone vortices for small assay samples consisting of only tens of cells. In our previous work of hydrodynamic cell capture, the laminar flow with abrupt expansion in a mircowell restricts the capture efficiency by diverting cells away from the capture site, which results in low capture rate. In this work, we used herringbone vortices to steer cells towards the capture site to facilitate cell capture efficiency. The proposed design achieves ∼65% capture efficiency when loading only 20, 40 and 60 MDA-MB-321 (breast cancer) cells. Compared to the capture efficiency of ∼35% without the herringbone, the proposed cell-focusing capture scheme demonstrates the feasibility for small samples analysis.
UR - http://www.scopus.com/inward/record.url?scp=84907364040&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907364040&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907364040
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 350
EP - 352
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
ER -