High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis

Yu Heng Cheng, Yu Chih Chen, Patrick Ingram, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a high-efficiency single-cell capture scheme utilizing herringbone vortices for small assay samples consisting of only tens of cells. In our previous work of hydrodynamic cell capture, the laminar flow with abrupt expansion in a mircowell restricts the capture efficiency by diverting cells away from the capture site, which results in low capture rate. In this work, we used herringbone vortices to steer cells towards the capture site to facilitate cell capture efficiency. The proposed design achieves ∼65% capture efficiency when loading only 20, 40 and 60 MDA-MB-321 (breast cancer) cells. Compared to the capture efficiency of ∼35% without the herringbone, the proposed cell-focusing capture scheme demonstrates the feasibility for small samples analysis.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages350-352
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Vortex flow
Laminar flow
Assays
Hydrodynamics

All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Cheng, Y. H., Chen, Y. C., Ingram, P., & Yoon, E. (2013). High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 350-352). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.
Cheng, Yu Heng ; Chen, Yu Chih ; Ingram, Patrick ; Yoon, Euisik. / High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 350-352 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
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title = "High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis",
abstract = "We present a high-efficiency single-cell capture scheme utilizing herringbone vortices for small assay samples consisting of only tens of cells. In our previous work of hydrodynamic cell capture, the laminar flow with abrupt expansion in a mircowell restricts the capture efficiency by diverting cells away from the capture site, which results in low capture rate. In this work, we used herringbone vortices to steer cells towards the capture site to facilitate cell capture efficiency. The proposed design achieves ∼65{\%} capture efficiency when loading only 20, 40 and 60 MDA-MB-321 (breast cancer) cells. Compared to the capture efficiency of ∼35{\%} without the herringbone, the proposed cell-focusing capture scheme demonstrates the feasibility for small samples analysis.",
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Cheng, YH, Chen, YC, Ingram, P & Yoon, E 2013, High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 1, Chemical and Biological Microsystems Society, pp. 350-352, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.

High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis. / Cheng, Yu Heng; Chen, Yu Chih; Ingram, Patrick; Yoon, Euisik.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 350-352 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Cheng YH, Chen YC, Ingram P, Yoon E. High efficiency single cell capture chip utilizing herringbone vortices for small sample analysis. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 350-352. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).