High performance, disposable hollow fiber flow field-flow fractionation for bacteria and cells. First application to deactivated Vibrio cholerae

Pierluigi Reschiglian, Barbara Roda, Andrea Zattoni, Byung Ryul Min, Myeong Hee Moon

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Interest in low-cost, analytical-scale, highly efficient, and sensitive separation methods for cells and bacteria has recently been increasing. Field-flow fractionation is well suited to the separation of different types of cells, including bacteria. High performance hollow fiber flow field-flow fractionation of such samples is demonstrated here for the first time with potentially disposable channels and high-sensitivity UV/Vis detectors. In this first application, hollow fiber flow field-flow fractionation is used to fractionate bacteria of biotechnological interest such as deactivated Vibrio cholerae, which are employed for whole-bacteria vaccine production. Quite short analysis times, high reproducibility, and low limits of detection are found. Retention of Vibrio cholerae is shown to depend on the mobile phase composition. Two serologically different Vibrio cholerae strains are partly distinguished by their fractogram profiles.

Original languageEnglish
Pages (from-to)490-498
Number of pages9
JournalJournal of Separation Science
Volume25
Issue number8
DOIs
Publication statusPublished - 2002 Jul 9

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Fractionation
Flow fields
Bacteria
Fibers
Ultraviolet detectors
Vaccines
Phase composition
Costs

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

Cite this

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abstract = "Interest in low-cost, analytical-scale, highly efficient, and sensitive separation methods for cells and bacteria has recently been increasing. Field-flow fractionation is well suited to the separation of different types of cells, including bacteria. High performance hollow fiber flow field-flow fractionation of such samples is demonstrated here for the first time with potentially disposable channels and high-sensitivity UV/Vis detectors. In this first application, hollow fiber flow field-flow fractionation is used to fractionate bacteria of biotechnological interest such as deactivated Vibrio cholerae, which are employed for whole-bacteria vaccine production. Quite short analysis times, high reproducibility, and low limits of detection are found. Retention of Vibrio cholerae is shown to depend on the mobile phase composition. Two serologically different Vibrio cholerae strains are partly distinguished by their fractogram profiles.",
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High performance, disposable hollow fiber flow field-flow fractionation for bacteria and cells. First application to deactivated Vibrio cholerae. / Reschiglian, Pierluigi; Roda, Barbara; Zattoni, Andrea; Min, Byung Ryul; Moon, Myeong Hee.

In: Journal of Separation Science, Vol. 25, No. 8, 09.07.2002, p. 490-498.

Research output: Contribution to journalArticle

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AU - Moon, Myeong Hee

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