High-rate mesophilic hydrogen production from food waste using hybrid immobilized microbiome

Ju Hyeong Jung, Young Bo Sim, Jong Hyun Baik, Jong Hun Park, Sang Hyoun Kim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


This study examined the feasibility of dark fermentative biohydrogen production from food waste using hybrid immobilization in mesophilic condition. Among four different organic loading rates (OLRs), the highest average hydrogen production rate (HPR) of 9.82 ± 0.30 L/L-d was found at an OLR of 74.7 g hexose/L-d, which was higher than reported values from particulate feedstock in mesophilic condition. The average hydrogen yield (HY) at the condition was 1.25 ± 0.04 mol H2/mol hexoseconsumed. Whereas the average HPR and HY at an OLR 80 g hexose/L-d were 5.82 ± 0.12 L/L-d and 0.64 ± 0.02 mol H2/mol hexoseconsumed, respectively. Metabolic flux analysis showed the low HY was concurrent with the highest propionic acid and homoacetogenis. Bacterial population was shift from Clostridium sp. to non-hydrogen producers including Bifidobacterium, Bacteriodes, Olsenella, Dysgonomonas, and Dialister sp.

Original languageEnglish
Article number124279
JournalBioresource technology
Publication statusPublished - 2021 Jan

Bibliographical note

Funding Information:
The research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science & ICT) (No. NRF-2019M3E6A1103839).

Publisher Copyright:
© 2020 Elsevier Ltd

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


Dive into the research topics of 'High-rate mesophilic hydrogen production from food waste using hybrid immobilized microbiome'. Together they form a unique fingerprint.

Cite this