Abstract
The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.
| Original language | English |
|---|---|
| Article number | 5402 |
| Journal | Nature communications |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2018 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Physics and Astronomy(all)
Cite this
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High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy. / Kang, Byungjun; Shin, Jisoo; Park, Hyun Ji; Rhyou, Chanryeol; Kang, Donyoung; Lee, Shin Jeong; Yoon, Young sup; Cho, Seung Woo; Lee, Hyungsuk.
In: Nature communications, Vol. 9, No. 1, 5402, 01.12.2018.Research output: Contribution to journal › Article
TY - JOUR
T1 - High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy
AU - Kang, Byungjun
AU - Shin, Jisoo
AU - Park, Hyun Ji
AU - Rhyou, Chanryeol
AU - Kang, Donyoung
AU - Lee, Shin Jeong
AU - Yoon, Young sup
AU - Cho, Seung Woo
AU - Lee, Hyungsuk
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.
AB - The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.
UR - http://www.scopus.com/inward/record.url?scp=85058891650&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85058891650&partnerID=8YFLogxK
U2 - 10.1038/s41467-018-07823-5
DO - 10.1038/s41467-018-07823-5
M3 - Article
C2 - 30573732
AN - SCOPUS:85058891650
VL - 9
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 5402
ER -