High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy

Byungjun Kang, Jisoo Shin, Hyun Ji Park, Chanryeol Rhyou, Donyoung Kang, Shin Jeong Lee, Young sup Yoon, Seung Woo Cho, Hyungsuk Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.

Original languageEnglish
Article number5402
JournalNature communications
Volume9
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

Fingerprint

cylindrical bodies
ischemia
therapy
Ischemia
Tissue
high resolution
cells
vessels
Therapeutics
secretions
stem cells
gene expression
Hydrogel
Endothelial cells
Human Umbilical Vein Endothelial Cells
Therapeutic Uses
Hyaluronic Acid
Drug Discovery
Stem cells
veins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Kang, Byungjun ; Shin, Jisoo ; Park, Hyun Ji ; Rhyou, Chanryeol ; Kang, Donyoung ; Lee, Shin Jeong ; Yoon, Young sup ; Cho, Seung Woo ; Lee, Hyungsuk. / High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy. In: Nature communications. 2018 ; Vol. 9, No. 1.
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High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy. / Kang, Byungjun; Shin, Jisoo; Park, Hyun Ji; Rhyou, Chanryeol; Kang, Donyoung; Lee, Shin Jeong; Yoon, Young sup; Cho, Seung Woo; Lee, Hyungsuk.

In: Nature communications, Vol. 9, No. 1, 5402, 01.12.2018.

Research output: Contribution to journalArticle

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