We have demonstrated the high-speed confocal fluorescence lifetime imaging microscopy (FLIM) by analog mean-delay (AMD) method. The AMD method is a new signal processing technique for calculation of fluorescence lifetime and it is very suitable for the high-speed confocal FLIM with good accuracy and photon economy. We achieved the acquisition speed of 7.7 frames per second for confocal FLIM imaging. Here, the highest photon detection rate for one pixel was larger than 125 MHz and averaged photon detection rate was more than 62.5 MHz. Based on our system, we successfully obtained a sequence of confocal fluorescence lifetime images of RBL-2H3 cell labeled with Fluo-3/AM and excited by 4αPDD (TRPV channel agonist) within one second.
|Title of host publication||Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII|
|Publication status||Published - 2010|
|Event||Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States|
Duration: 2010 Jan 23 → 2010 Jan 25
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Other||Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII|
|City||San Francisco, CA|
|Period||10/1/23 → 10/1/25|
Bibliographical noteFunding Information:
It is a pleasure to thank B. J. Wills for her HST composite quasar spectrum. We thank the referee R. Antonucci for helpful comments. We also thank the staff at Steward Observatory for flawless operation of the 2.3 m telescope during our runs. This work is supported by NSF grant 91-14087. D. C. H. and F. J. L. acknowledge additional support from JPL contract 959969. P. S. S. acknowledges additional support from NASA grant NAG 5-1630. R. M. C. is supported by the Jet Propulsion Laboratory, California Institute of Technology, which is operated under contract with NASA.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging