High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method

Youngjae Won, Donguk Kim, Wenzhong Yang, Dug Y. Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have demonstrated the high-speed confocal fluorescence lifetime imaging microscopy (FLIM) by analog mean-delay (AMD) method. The AMD method is a new signal processing technique for calculation of fluorescence lifetime and it is very suitable for the high-speed confocal FLIM with good accuracy and photon economy. We achieved the acquisition speed of 7.7 frames per second for confocal FLIM imaging. Here, the highest photon detection rate for one pixel was larger than 125 MHz and averaged photon detection rate was more than 62.5 MHz. Based on our system, we successfully obtained a sequence of confocal fluorescence lifetime images of RBL-2H3 cell labeled with Fluo-3/AM and excited by 4αPDD (TRPV channel agonist) within one second.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
DOIs
Publication statusPublished - 2010 May 10
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States
Duration: 2010 Jan 232010 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7568
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
CountryUnited States
CitySan Francisco, CA
Period10/1/2310/1/25

Fingerprint

Optical Imaging
Photons
Microscopy
Microscopic examination
Fluorescence
high speed
analogs
microscopy
Imaging techniques
life (durability)
fluorescence
photons
economy
signal processing
acquisition
Signal processing
Pixels
pixels
cells

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Won, Y., Kim, D., Yang, W., & Kim, D. Y. (2010). High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII [756816] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7568). https://doi.org/10.1117/12.840387
Won, Youngjae ; Kim, Donguk ; Yang, Wenzhong ; Kim, Dug Y. / High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method. Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. 2010. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Won, Y, Kim, D, Yang, W & Kim, DY 2010, High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII., 756816, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, San Francisco, CA, United States, 10/1/23. https://doi.org/10.1117/12.840387

High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method. / Won, Youngjae; Kim, Donguk; Yang, Wenzhong; Kim, Dug Y.

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. 2010. 756816 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7568).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Won Y, Kim D, Yang W, Kim DY. High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. 2010. 756816. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.840387