High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method

Youngjae Won; Donguk Kim; Wenzhong Yang; Dug Y. Kim

doi:10.1117/12.840387

High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method

Youngjae Won, Donguk Kim, Wenzhong Yang, Dug Y. Kim

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have demonstrated the high-speed confocal fluorescence lifetime imaging microscopy (FLIM) by analog mean-delay (AMD) method. The AMD method is a new signal processing technique for calculation of fluorescence lifetime and it is very suitable for the high-speed confocal FLIM with good accuracy and photon economy. We achieved the acquisition speed of 7.7 frames per second for confocal FLIM imaging. Here, the highest photon detection rate for one pixel was larger than 125 MHz and averaged photon detection rate was more than 62.5 MHz. Based on our system, we successfully obtained a sequence of confocal fluorescence lifetime images of RBL-2H3 cell labeled with Fluo-3/AM and excited by 4αPDD (TRPV channel agonist) within one second.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
DOIs	https://doi.org/10.1117/12.840387
Publication status	Published - 2010
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States Duration: 2010 Jan 23 → 2010 Jan 25

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7568
ISSN (Print)	1605-7422

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Country/Territory	United States
City	San Francisco, CA
Period	10/1/23 → 10/1/25

Bibliographical note

Funding Information:
It is a pleasure to thank B. J. Wills for her HST composite quasar spectrum. We thank the referee R. Antonucci for helpful comments. We also thank the staff at Steward Observatory for flawless operation of the 2.3 m telescope during our runs. This work is supported by NSF grant 91-14087. D. C. H. and F. J. L. acknowledge additional support from JPL contract 959969. P. S. S. acknowledges additional support from NASA grant NAG 5-1630. R. M. C. is supported by the Jet Propulsion Laboratory, California Institute of Technology, which is operated under contract with NASA.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.840387

Cite this

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title = "High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method",

abstract = "We have demonstrated the high-speed confocal fluorescence lifetime imaging microscopy (FLIM) by analog mean-delay (AMD) method. The AMD method is a new signal processing technique for calculation of fluorescence lifetime and it is very suitable for the high-speed confocal FLIM with good accuracy and photon economy. We achieved the acquisition speed of 7.7 frames per second for confocal FLIM imaging. Here, the highest photon detection rate for one pixel was larger than 125 MHz and averaged photon detection rate was more than 62.5 MHz. Based on our system, we successfully obtained a sequence of confocal fluorescence lifetime images of RBL-2H3 cell labeled with Fluo-3/AM and excited by 4αPDD (TRPV channel agonist) within one second.",

author = "Youngjae Won and Donguk Kim and Wenzhong Yang and Kim, {Dug Y.}",

note = "Funding Information: It is a pleasure to thank B. J. Wills for her HST composite quasar spectrum. We thank the referee R. Antonucci for helpful comments. We also thank the staff at Steward Observatory for flawless operation of the 2.3 m telescope during our runs. This work is supported by NSF grant 91-14087. D. C. H. and F. J. L. acknowledge additional support from JPL contract 959969. P. S. S. acknowledges additional support from NASA grant NAG 5-1630. R. M. C. is supported by the Jet Propulsion Laboratory, California Institute of Technology, which is operated under contract with NASA.; Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII ; Conference date: 23-01-2010 Through 25-01-2010",

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doi = "10.1117/12.840387",

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Won, Y, Kim, D, Yang, W & Kim, DY 2010, High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII., 756816, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, San Francisco, CA, United States, 10/1/23. https://doi.org/10.1117/12.840387

High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method. / Won, Youngjae; Kim, Donguk; Yang, Wenzhong et al.

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. 2010. 756816 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7568).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Kim, Dug Y.

N1 - Funding Information: It is a pleasure to thank B. J. Wills for her HST composite quasar spectrum. We thank the referee R. Antonucci for helpful comments. We also thank the staff at Steward Observatory for flawless operation of the 2.3 m telescope during our runs. This work is supported by NSF grant 91-14087. D. C. H. and F. J. L. acknowledge additional support from JPL contract 959969. P. S. S. acknowledges additional support from NASA grant NAG 5-1630. R. M. C. is supported by the Jet Propulsion Laboratory, California Institute of Technology, which is operated under contract with NASA.

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N2 - We have demonstrated the high-speed confocal fluorescence lifetime imaging microscopy (FLIM) by analog mean-delay (AMD) method. The AMD method is a new signal processing technique for calculation of fluorescence lifetime and it is very suitable for the high-speed confocal FLIM with good accuracy and photon economy. We achieved the acquisition speed of 7.7 frames per second for confocal FLIM imaging. Here, the highest photon detection rate for one pixel was larger than 125 MHz and averaged photon detection rate was more than 62.5 MHz. Based on our system, we successfully obtained a sequence of confocal fluorescence lifetime images of RBL-2H3 cell labeled with Fluo-3/AM and excited by 4αPDD (TRPV channel agonist) within one second.

AB - We have demonstrated the high-speed confocal fluorescence lifetime imaging microscopy (FLIM) by analog mean-delay (AMD) method. The AMD method is a new signal processing technique for calculation of fluorescence lifetime and it is very suitable for the high-speed confocal FLIM with good accuracy and photon economy. We achieved the acquisition speed of 7.7 frames per second for confocal FLIM imaging. Here, the highest photon detection rate for one pixel was larger than 125 MHz and averaged photon detection rate was more than 62.5 MHz. Based on our system, we successfully obtained a sequence of confocal fluorescence lifetime images of RBL-2H3 cell labeled with Fluo-3/AM and excited by 4αPDD (TRPV channel agonist) within one second.

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Y2 - 23 January 2010 through 25 January 2010

ER -

High-speed confocal fluorescence lifetime imaging microscopy by analog mean-delay method

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