High-speed image acquisition synchronized with the motion of Galvanometer scanner for confocal microscopy

Yoon Sung Bae, Sucbei Moon, Dug Y. Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We present a simple 2D image acquisition technique electronically implemented for laser scanning confocal microscope using galvanometer scanners. In order to synchronize image acquisition process with the movement of the galvanometer scanner, position signal of the mirror of the galvanometer scanner is used and manipulated for generating of sync-signals. This is achieved using an analog-digital converter to read a position signal from the scanner which tells about its position and to generate a trigger signal (or pixel clock) which tells the moment of digitizing the received analog signal from the photo-detector. This facilitates processing the image in synchronization with the actual motion of the scanning laser beam scanner. Image construction is performed by a video acquisition board (frame grabber). The newly developed scanning and image acquisition systems are implemented in a confocal microscope with fiber-optic components for compact configuration and flexible light path.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XV
DOIs
Publication statusPublished - 2008
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV - San Jose, CA, United States
Duration: 2008 Jan 212008 Jan 24

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6861
ISSN (Print)1605-7422

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV
CountryUnited States
CitySan Jose, CA
Period08/1/2108/1/24

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Bae, Y. S., Moon, S., & Kim, D. Y. (2008). High-speed image acquisition synchronized with the motion of Galvanometer scanner for confocal microscopy. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV [686116] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6861). https://doi.org/10.1117/12.763641